Application
Fig1: Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution. Positive control： Lane1: HepG2 Lane2: Human liver tissueApplication
Fig2: ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.| Product Name | AKR1C1 [5-F10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2-dihydrobenzene-1 antibody 2-diol dehydrogenase antibody 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody 20 ALPHA HSD antibody 20 alpha hydroxysteroid dehydrogenase antibody 20-alpha-HSD antibody 20-alpha-hydroxysteroid dehydrogenase antibody 20ALPHAHSD antibody 2ALPHAHSD antibody AK1C1 antibody AK1C1_HUMAN antibody AKR1C1 antibody Aldo keto reductase family 1 member C1 antibody aldo-keto reductase C antibody Aldo-keto reductase family 1 member C1 antibody Aldo-keto reductase family, 1 member 1 antibody C9 antibody Chlordecone reductase homolog antibody Chlordecone reductase homolog HAKRC antibody DD1 antibody DD1/DD2 antibody DDH antibody DDH1 antibody Dihydrodiol dehydrogenase 1 antibody Dihydrodiol dehydrogenase 1/2 antibody dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase antibody dihydrodiol dehydrogenase isoform DD1 antibody Dihydrodiol dehydrogenase, type 1 antibody H37 antibody HAKRC antibody HBAB antibody Hepatic dihydrodiol dehydrogenase antibody High affinity hepatic bile acid-binding protein antibody High-affinity hepatic bile acid-binding protein antibody Indanol dehydrogenase antibody MBAB antibody MGC8954 antibody Trans-1 antibody Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase antibody Type II 3 alpha hydroxysteroid dehydrogenase antibody |
| Product description | Human liver contains isoforms of dihydrodiol dehydrogenase (DD1, DD2, DD3 and DD4), which belong to the aldo-oxo reductase / aldo-keto reductase (AKR) superfamily, have 20α- or 3α-hydroxysteroid dehydrogenase AKD1C3 and 3α-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 is also designated as AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C4, CD, CHDR or AKR1C6 (in rodents). DD1 and DD2 are 20α-HSDs, DD3 and DD4 are the 3α-HSDs. The 20α-HSD catalyzes the reaction of progesterone to the inactive form 20α-hydroxyprogesterone. The 3α-HSD is a cytosolic, monomeric, NADPH-depen DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver. DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzene dihydrodiol and trans- naphthalenedihydrodiol. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:2,000-1:10,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 2-dihydrobenzene-1 antibody
2-diol dehydrogenase antibody
20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
20 ALPHA HSD antibody
20 alpha hydroxysteroid dehydrogenase antibody
20-alpha-HSD antibody
20-alpha-hydroxysteroid dehydrogenase antibody
20ALPHAHSD antibody
2ALPHAHSD antibody
AK1C1 antibody
AK1C1_HUMAN antibody
AKR1C1 antibody
Aldo keto reductase family 1 member C1 antibody
aldo-keto reductase C antibody
Aldo-keto reductase family 1 member C1 antibody
Aldo-keto reductase family 1 member 1 antibody C9 antibody Chlordecone reductase homolog antibody Chlordecone reductase homolog HAKRC antibody DD1 antibody DD1/DD2 antibody DDH antibody DDH1 antibody Dihydrodiol dehydrogenase 1 antibody Dihydrodiol dehydrogenase 1/2 antibody dihydrodiol dehydrogenase 1 20-alpha (3-alpha)-hydroxysteroid dehydrogenase antibody dihydrodiol dehydrogenase isoform DD1 antibody Dihydrodiol dehydrogenase type 1 antibody H37 antibody HAKRC antibody HBAB antibody Hepatic dihydrodiol dehydrogenase antibody High affinity hepatic bile acid-binding protein antibody High-affinity hepatic bile acid-binding protein antibody Indanol dehydrogenase antibody MBAB antibody MGC8954 antibody Trans-1 antibody Trans-1 2 dihydrobenzene 1 2 diol dehydrogenase antibody Type II 3 alpha hydroxysteroid dehydrogenase antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q04828 |
|---|
Application
Fig1: Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution. Positive control： Lane1: HepG2 Lane2: Human liver tissue
Application
Fig2: ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.| Positive Control | HepG2, human liver tissue, MCF-7, human pancreas tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:2,000-1:10,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
