Application
Fig1: Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution. Positive control： Lane1: NIH-3T3 Lane2: HepG2 Lane3: K562 Lane4: L929Application
Fig2: ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Grp75 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Grp75 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Grp75 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Grp75 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Grp75 antibody. Counter stained with hematoxylin.| Product Name | Grp75 [6-A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 75 kDa glucose regulated protein antibody 75 kDa glucose-regulated protein antibody CSA antibody Glucose Regulated Protein antibody Grp 75 antibody GRP-75 antibody GRP75 antibody GRP75_HUMAN antibody Heat shock 70 kDa protein 9 antibody Heat shock 70kD protein 9 antibody heat shock 70kDa protein 9 antibody Heat shock 70kDa protein 9B antibody Heat shock protein 74 kDa A antibody Heat shock protein A antibody Heat shock protein cognate 74 antibody Hsc74 antibody Hsp74 antibody Hsp74a antibody HSPA9 antibody Hspa9a antibody HSPA9B antibody MGC4500 antibody mitochondrial antibody Mortalin 2 antibody Mortalin antibody Mortalin perinuclear antibody Mortalin2 antibody MOT 2 antibody MOT antibody MOT2 antibody Mthsp70 antibody p66 mortalin antibody P66 MOT antibody PBP74 antibody Peptide binding protein 74 antibody Peptide-binding protein 74 antibody Stress 70 protein mitochondrial antibody Stress 70 protein mitochondrial precursor antibody Stress-70 protein antibody |
| Product description | The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. |
| Immunogen | Peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | 75 kDa glucose regulated protein antibody 75 kDa glucose-regulated protein antibody CSA antibody Glucose Regulated Protein antibody Grp 75 antibody GRP-75 antibody GRP75 antibody GRP75_HUMAN antibody Heat shock 70 kDa protein 9 antibody Heat shock 70kD protein 9 antibody heat shock 70kDa protein 9 antibody Heat shock 70kDa protein 9B antibody Heat shock protein 74 kDa A antibody Heat shock protein A antibody Heat shock protein cognate 74 antibody Hsc74 antibody Hsp74 antibody Hsp74a antibody HSPA9 antibody Hspa9a antibody HSPA9B antibody MGC4500 antibody mitochondrial antibody Mortalin 2 antibody Mortalin antibody Mortalin perinuclear antibody Mortalin2 antibody MOT 2 antibody MOT antibody MOT2 antibody Mthsp70 antibody p66 mortalin antibody P66 MOT antibody PBP74 antibody Peptide binding protein 74 antibody Peptide-binding protein 74 antibody Stress 70 protein mitochondrial antibody Stress 70 protein mitochondrial precursor antibody Stress-70 protein antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa |
| Cellular Localization | Mitochondrion. Nucleus. |
Application
Fig1: Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution. Positive control： Lane1: NIH-3T3 Lane2: HepG2 Lane3: K562 Lane4: L929
Application
Fig2: ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Grp75 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Grp75 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Grp75 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Grp75 antibody. Counter stained with hematoxylin.| Positive Control | HepG2, L929, K562, NIH-3T3, A549, SK-Br-3, rat epididymis tissue, mouse liver tissue, mouse oviduct tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
