Application
Fig1: Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution.Application
Fig2: ICC staining B Raf (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining B Raf (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-B Raf antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.| Product Name | B-Raf [F1-F4-E10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WB,IHC,ICC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | FLJ95109 antibody
94 kDa B raf protein antibody
B raf 1 antibody
B Raf proto oncogene serine threonine protein kinase antibody
B Raf proto oncogene serine/threonine kinase antibody B RAF1 antibody B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody BRAF 1 antibody BRAF antibody BRAF_HUMAN antibody BRAF1 antibody cRmil antibody MGC126806 antibody MGC138284 antibody Murine sarcoma viral (v-raf) oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody Oncogen BRAF antibody oncogene BRAF1 antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB 1 antibody RAFB1 antibody RMIL antibody Serine/threonine-protein kinase B-raf antibody v raf murine sarcoma viral oncogene homolog B antibody v raf murine sarcoma viral oncogene homolog B1 antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Cell membrane. |
Application
Fig1: Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution.
Application
Fig2: ICC staining B Raf (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining B Raf (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.| Positive Control | SiHa, LOVO, SH-SY5Y, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
