Application
Fig1: Western blot analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.Application
Fig2: ICC staining Glucagon (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Glucagon (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of PANC-1 cells with Glucagon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Glucagon [5F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCG antibody |
| Product description | Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2000 ICC |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | GCG antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa, additional band 55kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.
Application
Fig2: ICC staining Glucagon (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Glucagon (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of PANC-1 cells with Glucagon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Mouse kidney tissue lysate, LOVO, PANC-1, SH-SY5Y, mouse pancreas tissue, rat pancreas tissue. |
|---|---|
| Application Notes | WB:1:500-1:2000 ICC |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
