Application Fig1: ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD47 antibody. Counter stained with hematoxylin.
Application Fig3: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-CD47 antibody. Counter stained with hematoxylin.
Application Fig4: Flow cytometric analysis of SH-SY5Y cells with CD47 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
| Product Name | CD47 [1A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP). CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes. It may also be involved in membrane permeability changes following viral infection. CD47 is expressed on hemopoietic cells, epithelial cells, endothelial cells and fibroblasts and is strongly expressed in brain and mesenchymal cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | ICCIHCFC |
| ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Antigen identified by monoclonal antibody 1D8 antibody
Antigenic surface determinant protein OA3 antibody
CD 47 antibody
Cd47 antibody
CD47 antigen (Rh-related antigen integrin-associated signal transducer) antibody CD47 antigen antibody CD47 glycoprotein antibody CD47 molecule antibody CD47_HUMAN antibody IAP antibody Integrin Associated Protein antibody Integrin associated signal transducer antibody Integrin-associated protein antibody Leukocyte surface antigen CD47 antibody MER 6 antibody MER6 antibody OA 3 antibody OA3 antibody OTTHUMP00000041152 antibody OTTHUMP00000041153 antibody Protein MER6 antibody Rh related antigen antibody Surface antigen identified by monoclonal antibody 1D8 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
Application Fig1: ICC staining CD47 (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD47 antibody. Counter stained with hematoxylin.
Application Fig3: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-CD47 antibody. Counter stained with hematoxylin.
Application Fig4: Flow cytometric analysis of SH-SY5Y cells with CD47 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
| Positive Control | SH-SY5Y, human prostate tissue, mouse fallopian tube tissue. |
|---|---|
| Application Notes | ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
