Application
Fig1: Western blot analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRB2 antibody. Counter stained with hematoxylin.| Product Name | GRB2 [11-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein involved in signal transduction/cell communication. In humans, the GRB2 protein is encoded by the GRB2 gene. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WB,IHC,ICC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | Abundant SRC homology antibody Adapter protein GRB2 antibody ASH antibody Ash protein antibody EGFRBP GRB2 antibody Epidermal growth factor receptor binding protein antibody Epidermal growth factor receptor binding protein GRB2 antibody GRB 2 antibody GRB2 adapter protein antibody Grb2 antibody GRB2_HUMAN antibody Grb3 3 antibody Growth factor receptor bound protein 2 antibody Growth factor receptor bound protein 3 antibody Growth factor receptor-bound protein 2 antibody HT027 antibody MST084 antibody MSTP084 antibody NCKAP2 antibody OTTHUMP00000166096 antibody OTTHUMP00000166097 antibody OTTHUMP00000166098 antibody Protein ash antibody SEM5 antibody SH2/SH3 adapter GRB2 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Endosome, Nucleus, Golgi apparatus. |
Application
Fig1: Western blot analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRB2 antibody. Counter stained with hematoxylin.| Positive Control | 293, MCF-7, HepG2, rat brain tissue, human colon cancer tissue, |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
