Application
Fig1:Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody at 1/1,000 dilution. Lane 1: TF-1 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: U-937 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of THP-1 cells with CD11b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.IF-P
Species: Human, Site: Spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/50.| Product Name | CD11b Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. |
| Immunogen | Recombinant protein within Human CD11b aa 544-743 / 1,152. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 IF-P:1:50 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Protein A |
| Alternative Names | antigen CD11b (p170) antibody
Antigen CD11b p170 antibody CD11 antigen like family member B antibody CD11 antigen-like family member B antibody CD11b antibody CD11b/CD18 antibody CD49d antibody Cell surface glycoprotein MAC-1 subunit alpha antibody Complement component 3 receptor 3 subunit antibody Complement Component Receptor 3 Alpha antibody Complement receptor type 3 alpha subunit antibody CR 3 alpha chain (CR3A) antibody CR 3 alpha chain antibody CR-3 alpha chain antibody CR3 antibody CR3A antibody F730045J24Rik antibody Integrin Alpha M antibody Integrin alpha M chain antibody Integrin alpha-M antibody Integrin beta 2 alpha subunit antibody Integrin subunit alpha M antibody integrin alpha M (complement component 3 receptor 3 subunit) antibody ITAM_HUMAN antibody ITGAM antibody Leukocyte adhesion receptor MO1 antibody Ly-40 antibody MAC 1 antibody Mac-1a antibody MAC1 antibody Mac1 alpha subunit antibody MAC1A antibody Macrophage antigen alpha polypeptide antibody MGC117044 antibody Mo1 alpha subunit antibody MO1A antibody Neutrophil adherence receptor alpha M subunit antibody Neutrophil adherence receptor antibody SLEB6 antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa(Observed band size: 170 kDa) |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P11215 |
|---|
Application
Fig1:Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody at 1/1,000 dilution. Lane 1: TF-1 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: U-937 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of THP-1 cells with CD11b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
IF-P
Species: Human, Site: Spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/50.| Application Notes | WB:1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 IF-P:1:50 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
