Application
Fig1: Western blot analysis of UAP1 on Jurkat cell lysate using anti-UAP1 antibody at 1/500 dilution.Application
Fig2: ICC staining UAP1 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining UAP1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.| Product Name | UAP1 [4H1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,IHC,ICC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | AGX 1 antibody AGX antibody AGX-1 antibody AGX-2 antibody AGX1 antibody Antigen X antibody AntigenX antibody SPAG 2 antibody SPAG2 antibody Sperm associated antigen 2 antibody Sperm-associated antigen 2 antibody UAP 1 antibody uap1 antibody UAP1_HUMAN antibody UDP N acetylhexosamine pyrophosphorylase antibody UDP N acteylglucosamine pyrophosphorylase 1 antibody UDP-N-acetylglucosamine pyrophosphorylase antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of UAP1 on Jurkat cell lysate using anti-UAP1 antibody at 1/500 dilution.
Application
Fig2: ICC staining UAP1 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining UAP1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UAP1 antibody. Counter stained with hematoxylin.| Positive Control | Jurkat, A549, Hela, rat epididymis tissue, human lung cancer tissue, human colon cancer tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
