Application
Fig1: Western blot analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Gp83 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Gp83 antibody. Counter stained with hematoxylin.| Product Name | Gp83 [4D1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the ADAMs family of zinc proteases. These transmembrane proteins play roles in multiple processes including cell signaling, adhesion and migration. The encoded protein lacks protease activity and may play roles in protein-protein interactions and cell adhesion processes including sperm-egg fusion. Mutations in this gene may be involved in the progression of melanoma. May play an important role in male reproduction including sperm maturation and gonadotrope function. This is a non catalytic metalloprotease-like protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Molecular Weight(MW) | 85 kDa |
|---|---|
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Gp83 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Gp83 antibody. Counter stained with hematoxylin.| Positive Control | SH-SY-5Y, PC-3M, rat epididymis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
