Application Fig1: Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution. Positive control: Lane 1: Jurkat Lane 2: A431 Lane 3: K562
Application Fig2: ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig9: Flow cytometric analysis of K562 cells with PTBP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
| Product Name | PTBP1 [9A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. |
| Immunogen | Recombinant protein within human PTBP1 aa 320-550. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:5,000-1:10,000 IHC:1:50-1:100 ICC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | 57 kDa RNA binding protein PPTB 1 antibody 57 kDa RNA-binding protein PPTB-1 antibody Heterogeneous nuclear ribonucleoprotein I antibody Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody hnRNP I antibody HNRNP-I antibody HNRNPI antibody HNRPI antibody MGC10830 antibody MGC8461 antibody Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody Polypyrimidine Tract Binding Protein 1 antibody Polypyrimidine tract binding protein antibody Polypyrimidine tract-binding protein 1 antibody pPTB antibody PTB 1 antibody PTB 2 antibody PTB 3 antibody PTB 4 antibody PTB antibody PTB T antibody PTB1 antibody PTB2 antibody PTB3 antibody PTB4 antibody PTBP 1 antibody PTBP1 antibody PTBP1_HUMAN antibody PTBT antibody RNA Binding Protein antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
Application Fig1: Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution. Positive control: Lane 1: Jurkat Lane 2: A431 Lane 3: K562
Application Fig2: ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig3: ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig4: ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig7: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application Fig9: Flow cytometric analysis of K562 cells with PTBP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
| Positive Control | Jurkat, A431, K562, rat testis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, 293T, A549, SW480, K562. |
|---|---|
| Application Notes | WB:1:5,000-1:10,000 IHC:1:50-1:100 ICC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
