Application
Fig1: Western blot analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) cell lysates using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution.Application
Fig2: ICC staining Glucocorticoid Receptor alpha (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of A549 cells with Glucocorticoid Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Product Name | Glucocorticoid Receptor alpha Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active. |
| Immunogen | Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha. |
| Isotype | IgG2a |
|---|---|
| Host Species | Mouse |
| Tested Applications | FCICCIHCWB |
| WB:1:1,000-1:5,000 ICC:1:50-1:200: IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ |
| Purification | Protein G |
| Alternative Names | GCCR antibody GCR antibody Glucocorticoid receptor alpha isoform antibody Glucocorticoid receptor antibody GR antibody GRL antibody NR3C1 antibody Nuclear receptor subfamily 3 group C member 1 antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa |
| Cellular Localization | Nucleus. Cytoskeleton. Mitochondrion. |
| SwissProt ID | P04150 |
|---|
Application
Fig1: Western blot analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) cell lysates using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution.
Application
Fig2: ICC staining Glucocorticoid Receptor alpha (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of A549 cells with Glucocorticoid Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | Siha, A549, human spleen tissue, human breast cancer tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200: IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
