Application
Fig1: Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.Application
Fig2: ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Product Name | NFIB NF1B2 [1H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCAAT Box Binding Transcription Factor antibody CCAAT-box-binding transcription factor antibody CTF antibody HMGIC/NFIB antibody NF-I/B antibody NF1-B antibody NF1B antibody NF1B2 antibody NFI-B antibody NFI-RED antibody Nfib antibody NFIB_HUMAN antibody NFIB2 antibody NFIB3 antibody Nuclear factor 1 B-type antibody Nuclear factor 1/B antibody Nuclear Factor 1B antibody Nuclear Factor I B antibody Nuclear factor I/B antibody TGGCA Binding Protein antibody TGGCA-binding protein antibody TRANSCRIPTION FACTOR NFIB antibody |
| Product description | NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter. |
| Immunogen | Recombinant full length protein corresponding to human NFIB/NF1B2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | CCAAT Box Binding Transcription Factor antibody CCAAT-box-binding transcription factor antibody CTF antibody HMGIC/NFIB antibody NF-I/B antibody NF1-B antibody NF1B antibody NF1B2 antibody NFI-B antibody NFI-RED antibody Nfib antibody NFIB_HUMAN antibody NFIB2 antibody NFIB3 antibody Nuclear factor 1 B-type antibody Nuclear factor 1/B antibody Nuclear Factor 1B antibody Nuclear Factor I B antibody Nuclear factor I/B antibody TGGCA Binding Protein antibody TGGCA-binding protein antibody TRANSCRIPTION FACTOR NFIB antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | SH-SY-5Y, LO-2, rat heart tissue, human colon cancer tissue, human prostate tissue, human pancreas tissue, mouse liver tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
