Application
Fig1: Western blot analysis of PGP9.5 on A172 (1) and SHG-44 (2) using anti-PGP9.5 antibody at 1/500 dilution.Application
Fig2: ICC staining PGP9.5 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A549 cells with PGP9.5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Product Name | PGP9.5 [B1-5-6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epididymis luminal protein 117 antibody Epididymis secretory protein Li 53 antibody HEL 117 antibody HEL S 53 antibody NDGOA antibody Neuron cytoplasmic protein 9.5 antibody OTTHUMP00000218137 antibody OTTHUMP00000218139 antibody OTTHUMP00000218140 antibody OTTHUMP00000218141 antibody Park 5 antibody PARK5 antibody PGP 9.5 antibody PGP9.5 antibody PGP95 antibody Protein gene product 9.5 antibody Ubiquitin C terminal esterase L1 antibody Ubiquitin C terminal hydrolase antibody Ubiquitin C terminal hydrolase L1 antibody Ubiquitin carboxyl terminal esterase L1 antibody Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody Ubiquitin thioesterase L1 antibody Ubiquitin thiolesterase antibody Ubiquitin thiolesterase L1 antibody UCH-L1 antibody UCHL1 antibody UCHL1_HUMAN antibody |
| Product description | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. |
| Immunogen | Synthetic peptide within C-terminal human PGP9.5. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500 ICC:1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Epididymis luminal protein 117 antibody Epididymis secretory protein Li 53 antibody HEL 117 antibody HEL S 53 antibody NDGOA antibody Neuron cytoplasmic protein 9.5 antibody OTTHUMP00000218137 antibody OTTHUMP00000218139 antibody OTTHUMP00000218140 antibody OTTHUMP00000218141 antibody Park 5 antibody PARK5 antibody PGP 9.5 antibody PGP9.5 antibody PGP95 antibody Protein gene product 9.5 antibody Ubiquitin C terminal esterase L1 antibody Ubiquitin C terminal hydrolase antibody Ubiquitin C terminal hydrolase L1 antibody Ubiquitin carboxyl terminal esterase L1 antibody Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody Ubiquitin thioesterase L1 antibody Ubiquitin thiolesterase antibody Ubiquitin thiolesterase L1 antibody UCH-L1 antibody UCHL1 antibody UCHL1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. |
Application
Fig1: Western blot analysis of PGP9.5 on A172 (1) and SHG-44 (2) using anti-PGP9.5 antibody at 1/500 dilution.
Application
Fig2: ICC staining PGP9.5 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A549 cells with PGP9.5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | A172, SHG-44, A549, SH-SY5Y, rat cerebellum tissue, human kidney tissue, human pancreas tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
