Application
Fig1: Western blot analysis of ASS1 on HepG2 (1) and SiHa (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with ASS1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Product Name | ASS1 [11F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Argininosuccinate synthase 1 antibody Argininosuccinate synthase antibody Argininosuccinate synthetase 1 antibody ASS antibody Ass-1 antibody ass1 antibody ASSA antibody ASSY_HUMAN antibody Citrulline aspartate ligase antibody Citrullineaspartate ligase antibody CTLN1 antibody |
| Product description | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues. |
| Immunogen | Recombinant protein within human ASS1 aa 1-250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Argininosuccinate synthase 1 antibody Argininosuccinate synthase antibody Argininosuccinate synthetase 1 antibody ASS antibody Ass-1 antibody ass1 antibody ASSA antibody ASSY_HUMAN antibody Citrulline aspartate ligase antibody Citrullineaspartate ligase antibody CTLN1 antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Cytosol. |
Application
Fig1: Western blot analysis of ASS1 on HepG2 (1) and SiHa (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with ASS1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | HepG2, MCF-7, SiHa, human colon cancer tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
