Application
Fig1: Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATF6 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATF6 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of HepG2 cells with ATF6 antibody at 1/50 dilution (pink purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | ATF6 [8D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating transcription factor 6 alpha antibody Activating transcription factor 6 antibody ATF 6 antibody ATF6 alpha antibody ATF6 antibody ATF6-alpha antibody ATF6A antibody ATF6A_HUMAN antibody cAMP dependent transcription factor ATF 6 alpha antibody cAMP-dependent transcription factor ATF-6 alpha antibody Cyclic AMP dependent transcription factor ATF 6 alpha antibody DKFZp686P2194 antibody ESTM49 antibody FLJ21663 antibody Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody |
| Product description | Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina. |
| Immunogen | Recombinant protein within full length sequence of human ATF6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:100 FC：1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Activating transcription factor 6 alpha antibody Activating transcription factor 6 antibody ATF 6 antibody ATF6 alpha antibody ATF6 antibody ATF6-alpha antibody ATF6A antibody ATF6A_HUMAN antibody cAMP dependent transcription factor ATF 6 alpha antibody cAMP-dependent transcription factor ATF-6 alpha antibody Cyclic AMP dependent transcription factor ATF 6 alpha antibody DKFZp686P2194 antibody ESTM49 antibody FLJ21663 antibody Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Endoplasmic reticulum. Nucleus. |
Application
Fig1: Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of HepG2 cells with ATF6 antibody at 1/50 dilution (pink purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, rat brain tissue, human placenta tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:100 FC：1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
