Application
Fig1: ICC staining IL18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining IL18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IL18 in PC-12 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL18 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL18 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL18 antibody. Counter stained with hematoxylin.| Product Name | IL18 [AH16-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Iboctadekin antibody IFN gamma inducing factor antibody IFN-gamma-inducing factor antibody IGIF antibody IL 1 gamma antibody IL 18 antibody IL 1g antibody IL-1 gamma antibody IL-18 antibody IL1 gamma antibody IL18 antibody IL18 protein antibody IL18_HUMAN antibody IL1F4 antibody IL1g antibody IL1gamma antibody ILIF4 antibody Interferon gamma inducing factor antibody Interferon gamma-inducing factor antibody Interleukin 1 gamma antibody Interleukin 18 (interferon-gamma-inducing factor) antibody Interleukin 18 antibody Interleukin-1 gamma antibody Interleukin-18 antibody Interleukin18 antibody MGC12320 antibody |
| Product description | Interleukin-18 (IL-18) has been identified as a molecule that induces IFN-g production and enhances NK cell cytotoxicity. IL-18 receptor (IL-18R) is a type I membrane protein present in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta and heart, absent from brain, skeletal muscle, pancreas and kidney. IL-18R is present in Hodgkin's disease cell lines, and does not bind IL-1α or IL-1β. The association of IL-18 to IL-18R leads to activation of NFκB. At present two subunits of IL-18R have been characterized: IL-18Rα and IL-18Rβ. IL-18Rα has been described as the ligand-binding chain and IL-18Rβ as the signal-transduction chain. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICCIHCWBICC/IF |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Iboctadekin antibody IFN gamma inducing factor antibody IFN-gamma-inducing factor antibody IGIF antibody IL 1 gamma antibody IL 18 antibody IL 1g antibody IL-1 gamma antibody IL-18 antibody IL1 gamma antibody IL18 antibody IL18 protein antibody IL18_HUMAN antibody IL1F4 antibody IL1g antibody IL1gamma antibody ILIF4 antibody Interferon gamma inducing factor antibody Interferon gamma-inducing factor antibody Interleukin 1 gamma antibody Interleukin 18 (interferon-gamma-inducing factor) antibody Interleukin 18 antibody Interleukin-1 gamma antibody Interleukin-18 antibody Interleukin18 antibody MGC12320 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: ICC staining IL18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining IL18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IL18 in PC-12 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL18 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL18 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL18 antibody. Counter stained with hematoxylin.| Positive Control | A431, PC-12, Hela, human tonsil tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.07% Sodium Azide. |
