Application
Fig1: ICC staining NGAL in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining NGAL in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NGAL in SW480 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NGAL Tubulin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-NGAL antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded huamn liver tissue using anti-NGAL antibody. Counter stained with hematoxylin.| Product Name | NGAL [AH22-11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 24p3 antibody 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody Alpha 2 microglobulin related protein antibody HGNC:6526 antibody HNL antibody Lcn 2 antibody Lcn2 antibody Lipocalin-2 antibody Migration stimulating factor inhibitor antibody MSFI antibody Neutrophil gelatinase associated lipocalin antibody Neutrophil gelatinase associated lipocalin precursor antibody Neutrophil gelatinase-associated lipocalin antibody NGAL antibody NGAL_HUMAN antibody Oncogene 24p3 antibody Oncogenic lipocalin 24p3 antibody p25 antibody Siderocalin antibody siderocalin LCN2 antibody SV40 induced 24P3 protein antibody Uterocalin antibody |
| Product description | In addition to the monomeric mammalian progelatinase, two additional forms of progelatinase have been identified. The shorter of these additional forms is a covalently linked, disulfide-bridged protein that heterodimerizes with a short protein; an α2-Microglobulin-related protein known as neutrophil gelatinase-associated lipocalin (NGAL), which is moderately expressed in breast and lung tissues. NGAL belongs to the lipocalin family and has a high degree of similarity with rat α2-Microglobulin-related protein and mouse protein 24p3. NGAL is able to bind a derivative of the bacterial chemotactic peptide, suggesting that it has important immuno-modulatory functions. NGAL has been described as an inflammatory protein; it is released into the circulation as a result of the inflammatory activation of leukocytes initiated by the extra-corporeal circulation. In addition, NGAL synthesis is induced in epithelial cells in inflammatory and neoplastic colorectal diseases. In conclusion, NGAL may serve as a scavenger of bacterial products to function in the anti-inflammatory process. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWBICCIHC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 24p3 antibody 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody Alpha 2 microglobulin related protein antibody HGNC:6526 antibody HNL antibody Lcn 2 antibody Lcn2 antibody Lipocalin-2 antibody Migration stimulating factor inhibitor antibody MSFI antibody Neutrophil gelatinase associated lipocalin antibody Neutrophil gelatinase associated lipocalin precursor antibody Neutrophil gelatinase-associated lipocalin antibody NGAL antibody NGAL_HUMAN antibody Oncogene 24p3 antibody Oncogenic lipocalin 24p3 antibody p25 antibody Siderocalin antibody siderocalin LCN2 antibody SV40 induced 24P3 protein antibody Uterocalin antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P80188 |
|---|
Application
Fig1: ICC staining NGAL in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining NGAL in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NGAL in SW480 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NGAL Tubulin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-NGAL antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded huamn liver tissue using anti-NGAL antibody. Counter stained with hematoxylin.| Positive Control | A431, NIH-3T3, SW480, human stomach cancer tissue, human spleen tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.11% Sodium Azide. |
