Application
Fig1: ICC staining interferon gamma in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining interferon gamma in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.| Product Name | γ-IFN [AH33-31] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IFG IFI IFN gamma IFN Immune IFNG Interferon gamma Interferon Gamma Precursor Macrophage Activating Factor MAF T Cell Interferon Type II Interferon |
| Product description | IFN-γ induces a variety of biological responses, such as antiviral, antiproliferative and immunomodulatory activity in sensitive cells. Activation of the IFN-γ receptor (IFN-γR) leads to autophosphorylation of the Janus kinases JAK1 and JAK2, and the nuclear translocation of the transcription factors Stat1α p91 and Stat1β p84. The IFN-γR is composed of at least two chains, designated IFN-γRα and IFN-γRβ, respectively. Although expression of IFN-γRα is sufficient for ligand binding, it alone does not confer responsiveness to IFN-γ. Concomitant expression of IFN-γRα and IFN-γRβ is required for transcriptional activation of IFN-γ-inducible genes. The IFN-γRβ chain, also called AF-1, is 332 and 337 amino acids in length in mouse and human, respectively, and may represent the signal transducing component of the IFN-γR. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWBICCIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | IFG IFI IFN gamma IFN Immune IFNG Interferon gamma Interferon Gamma Precursor Macrophage Activating Factor MAF T Cell Interferon Type II Interferon |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P01579 |
|---|
Application
Fig1: ICC staining interferon gamma in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining interferon gamma in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- interferon gamma antibody. Counter stained with hematoxylin.| Positive Control | 293T, A549, human tonsil tissue, human lung tissue, mouse colon tissue, mouse brain tissue, mouse testis tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.15% Sodium Azide. |
