Application
Fig1: Western blot analysis of IL6 on human spleen cell lysates using anti-IL6 antibody at 1/500 dilution.Application
Fig2: ICC staining IL6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IL6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining IL6 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL6 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL6 antibody. Counter stained with hematoxylin.| Product Name | IL6 [AH21-63] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin BSF 2 antibody B cell differentiation factor antibody B cell stimulatory factor 2 antibody B-cell stimulatory factor 2 antibody BSF 2 antibody BSF-2 antibody BSF2 antibody CDF antibody CTL differentiation factor antibody Hepatocyte stimulatory factor antibody HGF antibody HSF antibody Hybridoma growth factor antibody Hybridoma growth factor Interferon beta-2 antibody IFN-beta-2 antibody IFNB2 antibody IL 6 antibody IL-6 antibody IL6 antibody IL6_HUMAN antibody Interferon beta 2 antibody Interferon beta-2 antibody Interleukin 6 antibody Interleukin 6 (interferon beta 2) antibody Interleukin BSF 2 antibody Interleukin-6 antibody |
| Product description | Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWBICCIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Interleukin BSF 2 antibody B cell differentiation factor antibody B cell stimulatory factor 2 antibody B-cell stimulatory factor 2 antibody BSF 2 antibody BSF-2 antibody BSF2 antibody CDF antibody CTL differentiation factor antibody Hepatocyte stimulatory factor antibody HGF antibody HSF antibody Hybridoma growth factor antibody Hybridoma growth factor Interferon beta-2 antibody IFN-beta-2 antibody IFNB2 antibody IL 6 antibody IL-6 antibody IL6 antibody IL6_HUMAN antibody Interferon beta 2 antibody Interferon beta-2 antibody Interleukin 6 antibody Interleukin 6 (interferon beta 2) antibody Interleukin BSF 2 antibody Interleukin-6 antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of IL6 on human spleen cell lysates using anti-IL6 antibody at 1/500 dilution.
Application
Fig2: ICC staining IL6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IL6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining IL6 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL6 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL6 antibody. Counter stained with hematoxylin.| Positive Control | Hela, A549, PC-3M, human spleen cell lysates, human spleen tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.20% Sodium Azide. |
