Application
Fig1: ICC staining TSH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining TSH in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TSH antibody. Counter stained with hematoxylin.| Product Name | TSH [AH21-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CG ALPHA antibody CGA antibody Chorionic gonadotropin alpha polypeptide antibody FSHA antibody Glycoprotein hormones alpha polypeptide antibody GPHA1 antibody Thyroid stimulating hormone beta subunit antibody Thyrotropin beta subunit antibody TSH beta antibody TSHA antibody TSHB antibody |
| Product description | Various hormones are secreted from the anterior pituitary during development and growth, including thyroid-stimulating hormone (TSH, also known as thyrotropin), follicle-stimulating hormone (FSH) and leutinizing hormone (LH). TSH, FSH, and LH are heterodimers formed from a common α chain and a unique β chain. TSH is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSHβ is regulated by thyroid hormone (T3) and various retinoid compounds. TSHβ binds to the thyroid-stimulating hormone receptor (TSHR), which plays a major role in regulating thyroid function. TSHR is thought to exist in multiple glycosylation states. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWBIHCICC |
| WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | CG ALPHA antibody CGA antibody Chorionic gonadotropin alpha polypeptide antibody FSHA antibody Glycoprotein hormones alpha polypeptide antibody GPHA1 antibody Thyroid stimulating hormone beta subunit antibody Thyrotropin beta subunit antibody TSH beta antibody TSHA antibody TSHB antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: ICC staining TSH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining TSH in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TSH antibody. Counter stained with hematoxylin.| Positive Control | HepG2, JAR, mouse placenta tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 ELISA:1:5,000-1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.23% Sodium Azide. |
