Application
Fig1: Western blot analysis of EIF5A on human EIF5A recombinant protein using anti-EIF5A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of EIF5A on HEK293 (1) and EIF5A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF5A antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EIF5A antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela cells with EIF5A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EIF5A [6E-F10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | eIF 4D antibody eIF 5A 1 antibody EIF 5A antibody eIF 5A1 antibody eIF-4D antibody eIF-5A antibody eIF-5A-1 antibody eIF-5A1 antibody eIF4D antibody eif5a antibody EIF5A1 antibody eIF5AI antibody Eukaryotic initiation factor 5A antibody Eukaryotic initiation factor 5A isoform 1 antibody Eukaryotic translation initiation factor 5A 1 antibody Eukaryotic translation initiation factor 5A antibody Eukaryotic translation initiation factor 5A-1 antibody IF5A1_HUMAN antibody MGC104255 antibody MGC99547 antibody Rev binding factor antibody Rev-binding factor antibody uORF A antibody uORF antibody |
| Product description | In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A1, maps to chromosome 17p131 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A1 localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A1 contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A1's biochemical activity and for cellular proliferative signaling. In addition, eIF5A1 is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A1 interaction with Rev leads to a block of the viral replication cycle. A highly-conserved protein that is found in all living organisms, eIF5A2 (eukaryotic translation initiation factor 5A-2) is a 153 amino acid protein that has 94% sequence similarity to eIF5A1 and also shares the hypusine residue. Amplification of the gene encoding eIF5A2 is observed in ovarian carcinomas and overexpression of eIF5A2 is linked to advanced stages of ovarian cancers. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFCICC/IF |
| WB:1:500-1:2,000 IHC:1:200-1:500 FC:1:200-1:400 ELISA:1:10,000 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | eIF 4D antibody eIF 5A 1 antibody EIF 5A antibody eIF 5A1 antibody eIF-4D antibody eIF-5A antibody eIF-5A-1 antibody eIF-5A1 antibody eIF4D antibody eif5a antibody EIF5A1 antibody eIF5AI antibody Eukaryotic initiation factor 5A antibody Eukaryotic initiation factor 5A isoform 1 antibody Eukaryotic translation initiation factor 5A 1 antibody Eukaryotic translation initiation factor 5A antibody Eukaryotic translation initiation factor 5A-1 antibody IF5A1_HUMAN antibody MGC104255 antibody MGC99547 antibody Rev binding factor antibody Rev-binding factor antibody uORF A antibody uORF antibody |
|---|---|
| Molecular Weight(MW) | 16.8 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Endoplasmic reticulum membrane |
| SwissProt ID | P63241 |
|---|
Application
Fig1: Western blot analysis of EIF5A on human EIF5A recombinant protein using anti-EIF5A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of EIF5A on HEK293 (1) and EIF5A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF5A antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EIF5A antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela cells with EIF5A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human EIF5A recombinant protein, EIF5A-hIgGFc transfected HEK293 cell lysate, human colon cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:200-1:500 FC:1:200-1:400 ELISA:1:10,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
