Application
Fig1: Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CD68 on different cell lysate using anti-CD68 antibody at 1/1,000 dilution. Positive control： Line1: U937 Line1: Hela Line2: HepG2 Line3: JurkatApplication
Fig4: ICC staining CD68 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CD68 [F3-D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 68 antibody CD68 antibody CD68 antigen antibody CD68 molecule antibody CD68_HUMAN antibody DKFZp686M18236 antibody gp11 antibody Gp110 antibody LAMP4 antibody Macrophage antigen CD68 (microsialin) antibody MACROPHAGE ANTIGEN CD68 antibody Macrosialin antibody SCARD1 antibody Scavenger receptor class D member 1 antibody |
| Product description | CD68, which is homologous to the mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins (LGPs) that includes lamp-1 and lamp-2. CD68 is found in cytoplasmic granules and in the cytoplasm of various non-hematopoietic tissues including liver and kidney tubules and glomeruli. CD68 is also found, to a lesser extent, on the surface of macrophages, monocytes, neutrophils, basophils and large lymphocytes. LGPs are major components of lysosomal membranes and may act to protect the membranes from attack by hydrolases. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 ICC:1:200-1:1,000 IHC:1:200-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD 68 antibody CD68 antibody CD68 antigen antibody CD68 molecule antibody CD68_HUMAN antibody DKFZp686M18236 antibody gp11 antibody Gp110 antibody LAMP4 antibody Macrophage antigen CD68 (microsialin) antibody MACROPHAGE ANTIGEN CD68 antibody Macrosialin antibody SCARD1 antibody Scavenger receptor class D member 1 antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cell membrane and Endosome membrane. Lysosome membrane. |
| SwissProt ID | P34810 |
|---|
Application
Fig1: Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CD68 on different cell lysate using anti-CD68 antibody at 1/1,000 dilution. Positive control： Line1: U937 Line1: Hela Line2: HepG2 Line3: Jurkat
Application
Fig4: ICC staining CD68 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CD68 recombinant protein, CD68-hIgGFc transfected HEK293 cell lysate, U937, Hela, HepG2, human endometrial cancer tissue, human bladder cancer tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:1,000 IHC:1:200-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
