Application
Fig1: Western blot analysis of RAD52 on human RAD52 recombinant protein using anti-RAD52 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAD52 on HEK293 (1) and RAD52-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD52 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of RAD52 on different cell lysate using anti-RAD52 antibody at 1/1,000 dilution. Positive control： Line1: HepG2 Line2: MCF-7 Line3: MCF-7 Line4: C6Application
Fig4: ICC staining RAD52 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of MCF-7 cells with LAMP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAD52 [E11-E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA repair protein RAD52 antibody DNA repair protein RAD52 antibody DNA repair protein RAD52 homolog antibody RAD 52 antibody Rad52 antibody RAD52 homolog (S. cerevisiae) antibody RAD52 homolog (S. cerevisiae) antibody RAD52 homolog antibody RAD52_HUMAN antibody Recombination protein RAD52 antibody Recombination protein RAD52 antibody Rhabdomyosarcoma antigen MU RMS 40.23 antibody |
| Product description | Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | DNA repair protein RAD52 antibody DNA repair protein RAD52 antibody DNA repair protein RAD52 homolog antibody RAD 52 antibody Rad52 antibody RAD52 homolog (S. cerevisiae) antibody RAD52 homolog (S. cerevisiae) antibody RAD52 homolog antibody RAD52_HUMAN antibody Recombination protein RAD52 antibody Recombination protein RAD52 antibody Rhabdomyosarcoma antigen MU RMS 40.23 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P43351 |
|---|
Application
Fig1: Western blot analysis of RAD52 on human RAD52 recombinant protein using anti-RAD52 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAD52 on HEK293 (1) and RAD52-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD52 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of RAD52 on different cell lysate using anti-RAD52 antibody at 1/1,000 dilution. Positive control： Line1: HepG2 Line2: MCF-7 Line3: MCF-7 Line4: C6
Application
Fig4: ICC staining RAD52 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of MCF-7 cells with LAMP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAD52 recombinant protein, RAD52-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
