Application
Fig1: Western blot analysis of CaMKII gamma on human CaMKII gamma recombinant protein using anti- CaMKII gamma antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CaMKII gamma on HEK293 (1) and CaMKII gamma -hIgGFc transfected HEK293 (2) cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CaMKII gamma on different cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution. Positive control： Line1: PC-12 Line2: Jurkat Line3: T47D Line4:HepG2Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- CaMKII gamma antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- CaMKII gamma antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with CaMKII gamma antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CAMK2G [10C-G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody CaM kinase II subunit alpha antibody CaMK-II subunit alpha antibody CAMK2 antibody Camk2a antibody CAMK2B antibody CAMK2D antibody CAMK2G antibody CAMKA antibody KCC2A_HUMAN antibody |
| Product description | dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:200-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody CaM kinase II subunit alpha antibody CaMK-II subunit alpha antibody CAMK2 antibody Camk2a antibody CAMK2B antibody CAMK2D antibody CAMK2G antibody CAMKA antibody KCC2A_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Sarcoplasmic reticulum membrane. |
Application
Fig1: Western blot analysis of CaMKII gamma on human CaMKII gamma recombinant protein using anti- CaMKII gamma antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CaMKII gamma on HEK293 (1) and CaMKII gamma -hIgGFc transfected HEK293 (2) cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CaMKII gamma on different cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution. Positive control： Line1: PC-12 Line2: Jurkat Line3: T47D Line4:HepG2
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- CaMKII gamma antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- CaMKII gamma antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with CaMKII gamma antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CaMKII gamma recombinant protein, CaMKII gamma -hIgGFc transfected HEK293 cell lysate, PC-12, Jurkat, T47D, HepG2, human prostate cancer tissue, human bladder cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:200-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
