Application
Fig1: Western blot analysis of RAN on human RAN recombinant protein using anti-RAN antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAN on HEK293 (1) and RAN-hIgGFc transfected HEK293 (2) cell lysate using anti-RAN antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of RAN on different cell lysate using anti-RAN antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: NIH/3T3 Line3: A431 Line4: C6 Line5: Jurkat Line6: Hela Line7: COS7 Line8: JurkatApplication
Fig4: ICC staining RAN (green) and Actin filaments (red) in GC-7901 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining RAN (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig6: ICC staining RANRAN (green) and Actin filaments (red) in HepG2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RAN antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RAN antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with RAN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAN [A1-6D] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Androgen receptor associated protein 24 antibody Androgen receptor-associated protein 24 antibody ARA 24 antibody ARA24 antibody Gsp1 antibody GTP binding nuclear protein RAN antibody GTP-binding nuclear protein Ran antibody GTPase Ran antibody Guanosine triphosphatase Ran antibody LPS antibody OK/SW-cl.81 antibody ran antibody RAN member RAS oncogene family antibody RAN_HUMAN antibody RanGTPase antibody Ras like protein TC4 antibody Ras related nuclear protein antibody Ras-like protein TC4 antibody Ras-related nuclear protein antibody RASL2 8 antibody TC 4 antibody TC4 antibody |
| Product description | The small Ras-related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The Ran BP2 protein has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 ICC:1:200-1:500 IHC:1:200-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 2mg/ml |
| Alternative Names | Androgen receptor associated protein 24 antibody Androgen receptor-associated protein 24 antibody ARA 24 antibody ARA24 antibody Gsp1 antibody GTP binding nuclear protein RAN antibody GTP-binding nuclear protein Ran antibody GTPase Ran antibody Guanosine triphosphatase Ran antibody LPS antibody OK/SW-cl.81 antibody ran antibody RAN member RAS oncogene family antibody RAN_HUMAN antibody RanGTPase antibody Ras like protein TC4 antibody Ras related nuclear protein antibody Ras-like protein TC4 antibody Ras-related nuclear protein antibody RASL2 8 antibody TC 4 antibody TC4 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Melanosome. |
Application
Fig1: Western blot analysis of RAN on human RAN recombinant protein using anti-RAN antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAN on HEK293 (1) and RAN-hIgGFc transfected HEK293 (2) cell lysate using anti-RAN antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of RAN on different cell lysate using anti-RAN antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: NIH/3T3 Line3: A431 Line4: C6 Line5: Jurkat Line6: Hela Line7: COS7 Line8: Jurkat
Application
Fig4: ICC staining RAN (green) and Actin filaments (red) in GC-7901 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining RAN (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: ICC staining RANRAN (green) and Actin filaments (red) in HepG2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RAN antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-RAN antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with RAN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAN recombinant protein, RAN-hIgGFc transfected HEK293 cell lysate, Hela, NIH/3T3, A431, C6, Jurkat, Hela, COS7, and Jurkat cell lysate, GC-7901, HepG2, human cervical cancer tissue, human stomach cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:500 IHC:1:200-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
