Application
Fig1: Western blot analysis of Histone H4 on THP-1 cell lysate using anti- Histone H4 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Histone H4 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using anti- Histone H4 antibody.Application
Fig4:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti- Histone H4 antibody.Application
Fig5: Flow cytometric analysis of Raji cells with Histone H4 antiboy at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | Histone H4 [H2-E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4.k antibody H4/a antibody H4/b antibody H4/c antibody H4/d antibody H4/e antibody H4/g antibody H4/h antibody H4/I antibody H4/j antibody H4/k antibody H4/m antibody H4/n antibody H4/p antibody H4_HUMAN antibody H4F2 antibody H4F2iii antibody H4F2iv antibody H4FA antibody H4FB antibody H4FC antibody H4FD antibody H4FE antibody H4FG antibody H4FH antibody H4FI antibody H4FJ antibody H4FK antibody H4FM antibody H4FN antibody H4M antibody HIST1H4A antibody HIST1H4B antibody HIST1H4C antibody HIST1H4D antibody HIST1H4E antibody HIST1H4F antibody HIST1H4H antibody HIST1H4I antibody HIST1H4J antibody HIST1H4K antibody HIST1H4L antibody HIST2H4 antibody HIST2H4A antibody Hist4h4 antibody Histone 1 H4a antibody Histone 1 H4b antibody Histone 1 H4c antibody Histone 1 H4d antibody Histone 1 H4e antibody Histone 1 H4f antibody Histone 1 H4h antibody Histone 1 H4i antibody Histone 1 H4j antibody Histone 1 H4k antibody Histone 1 H4l antibody Histone 2 H4a antibody histone 4 H4 antibody Histone H4 antibody MGC24116 antibody |
| Product description | In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:200-1:500 IHC:1:200-1:500 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4.k antibody H4/a antibody H4/b antibody H4/c antibody H4/d antibody H4/e antibody H4/g antibody H4/h antibody H4/I antibody H4/j antibody H4/k antibody H4/m antibody H4/n antibody H4/p antibody H4_HUMAN antibody H4F2 antibody H4F2iii antibody H4F2iv antibody H4FA antibody H4FB antibody H4FC antibody H4FD antibody H4FE antibody H4FG antibody H4FH antibody H4FI antibody H4FJ antibody H4FK antibody H4FM antibody H4FN antibody H4M antibody HIST1H4A antibody HIST1H4B antibody HIST1H4C antibody HIST1H4D antibody HIST1H4E antibody HIST1H4F antibody HIST1H4H antibody HIST1H4I antibody HIST1H4J antibody HIST1H4K antibody HIST1H4L antibody HIST2H4 antibody HIST2H4A antibody Hist4h4 antibody Histone 1 H4a antibody Histone 1 H4b antibody Histone 1 H4c antibody Histone 1 H4d antibody Histone 1 H4e antibody Histone 1 H4f antibody Histone 1 H4h antibody Histone 1 H4i antibody Histone 1 H4j antibody Histone 1 H4k antibody Histone 1 H4l antibody Histone 2 H4a antibody histone 4 H4 antibody Histone H4 antibody MGC24116 antibody |
|---|---|
| Molecular Weight(MW) | 11.4 kDa |
| Cellular Localization | Nucleus. Chromosome. |
| SwissProt ID | P62805 |
|---|
Application
Fig1: Western blot analysis of Histone H4 on THP-1 cell lysate using anti- Histone H4 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Histone H4 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using anti- Histone H4 antibody.
Application
Fig4:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti- Histone H4 antibody.
Application
Fig5: Flow cytometric analysis of Raji cells with Histone H4 antiboy at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | THP-1 cell lysate, Hela, paraffin-embedded rectum cancer tissues, paraffin-embedded stomach cancer tissues, Raji. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:500 IHC:1:200-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
