Application
Fig1: Western blot analysis of HDAC4 on human HDAC4 recombinant protein using anti-HDAC4 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HDAC4 on HEK293 (1) and HDAC4-hIgGFc transfected HEK293 (2) cell lysate using anti-HDAC4 antibody at 1/1,000 dilution.Application
Fig3: ICC staining HDAC4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with HDAC4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | HDAC4 [2E-6E] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AHO3 antibody BDMR antibody EC 3.5.1.98 antibody HA6116 antibody HD 4 antibody HD4 antibody HDAC 4 antibody HDAC A antibody HDAC4 antibody HDAC4_HUMAN antibody HDACA antibody Histone deacetylase 4 antibody Histone Deacetylase A antibody KIAA0288 antibody |
| Product description | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBP associated factor), HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:200-1:500 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AHO3 antibody BDMR antibody EC 3.5.1.98 antibody HA6116 antibody HD 4 antibody HD4 antibody HDAC 4 antibody HDAC A antibody HDAC4 antibody HDAC4_HUMAN antibody HDACA antibody Histone deacetylase 4 antibody Histone Deacetylase A antibody KIAA0288 antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. |
| SwissProt ID | P56524 |
|---|
Application
Fig1: Western blot analysis of HDAC4 on human HDAC4 recombinant protein using anti-HDAC4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HDAC4 on HEK293 (1) and HDAC4-hIgGFc transfected HEK293 (2) cell lysate using anti-HDAC4 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining HDAC4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with HDAC4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human HDAC4 recombinant protein, HDAC4-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
