Application
Fig1: Western blot analysis of Gemin 3 on human Germin 3 recombinant protein using anti-Germin 3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Germin 3 on HEK293 (1) and Germin 3-hIgGFc transfected HEK293 (2) cell lysat using anti- Germin 3 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of Germin 3 on different cell lysat using anti- Germin 3 antibody at 1/1,000 dilution. Positive control： Line1: Jurkat Line2: Hela Line3: NTERA-2 Line4: HL7702 Line5:K562 Line6:C6Application
Fig4: ICC staining Germin 3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with Germin 3 antibody at 1/50 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | DDX20 [F9-6C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Component of gems 3 antibody DDX 20 antibody ddx20 antibody DDX20_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody DEAD box protein 20 antibody DEAD box protein DP 103 antibody DEAD box protein DP103 antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody DEAD/H BOX 20 antibody DKFZP434H052 antibody DP 103 antibody DP103 antibody Gemin-3 antibody Gemin3 antibody Probable ATP dependent RNA helicase DDX20 antibody Probable ATP-dependent RNA helicase ddx20 antibody SMN interacting protein antibody |
| Product description | Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2. It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. It is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies). Gemin3 also interacts with SmB, SmD2 and SmD3. It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins. Gemin3 is a putative RNA helicase and shows ATPase activity. It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | Component of gems 3 antibody DDX 20 antibody ddx20 antibody DDX20_HUMAN antibody DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody DEAD box protein 20 antibody DEAD box protein DP 103 antibody DEAD box protein DP103 antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody DEAD/H BOX 20 antibody DKFZP434H052 antibody DP 103 antibody DP103 antibody Gemin-3 antibody Gemin3 antibody Probable ATP dependent RNA helicase DDX20 antibody Probable ATP-dependent RNA helicase ddx20 antibody SMN interacting protein antibody |
|---|---|
| Molecular Weight(MW) | 92 kDa |
| Cellular Localization | Cytoplasm. Nucleu. |
Application
Fig1: Western blot analysis of Gemin 3 on human Germin 3 recombinant protein using anti-Germin 3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Germin 3 on HEK293 (1) and Germin 3-hIgGFc transfected HEK293 (2) cell lysat using anti- Germin 3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Germin 3 on different cell lysat using anti- Germin 3 antibody at 1/1,000 dilution. Positive control： Line1: Jurkat Line2: Hela Line3: NTERA-2 Line4: HL7702 Line5:K562 Line6:C6
Application
Fig4: ICC staining Germin 3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with Germin 3 antibody at 1/50 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Germin 3 recombinant protein, Germin 3-hIgGFc transfected HEK293 cell lysat, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
