Application
Fig1: Western blot analysis of HOXA9 on human HOXA9 recombinant protein using anti-HOXA9 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HOXA9 on HEK293 (1) and HOXA9-hIgGFc transfected HEK293 (2) cell lysate using anti-HOXA9 antibody at 1/1,000 dilution.Application
Fig3: ICC staining HOXA9 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with HOXA9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | HOXA9 [6C-7C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABD-B antibody Abd-B, drosophila, homolog of antibody D6a9 antibody Homeobox 1G antibody Homeobox A9 antibody Homeobox protein Hox-1.7 antibody Homeobox protein Hox-1G antibody Homeobox protein Hox-A9 antibody Homeodomain protein HOXA9 antibody Hox-1.7 antibody Hox-1.7, mouse, homolog of antibody HOX1 antibody Hox1.7 antibody Hox1G antibody Hox1r5 antibody Hoxa-9 antibody Hoxa7 antibody HOXA9 antibody HOXA9/MSI2 fusion gene, included antibody HOXA9/NUP98 fusion gene, included antibody HXA9_HUMAN antibody MGC1934 antibody |
| Product description | The HOX homeobox genes encode proteins that play a role in embryonic development. The HOXA9 gene encodes a class I homeodomain protein, which is expressed in normal adult and fetal thymic tissue, and may play a role in regulating early differentiation of thymocytes. The HOXA9 homeodomain protein cooperatively binds consensus DNA sequences with Meis1 and Pbx1. In addition, the HOXA9 protein, along with the MEIS1 and Pbx 1 proteins, have been implicated in leukemic transformation in both mice and humans. Furthermore, overexpression of both HoxA9 and Meis1 in primary bone marrow cells in syngenic mice induced growth factor-dependent acute myeloid leukemia (AML). Chromosomal translocation of t(7;11)(p15;p15) has been demonstrated in patients with human AML and chronic myelogenous leukemia (CML), resulting in the fusion gene NUP98-HOXA9. Mice transplanted with bone marrow cells expressing NUP98-NOXA9 acquire a myeloproliferative disease (MPD) which ultimately degrades to AML. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | ABD-B antibody
Abd-B drosophila homolog of antibody D6a9 antibody Homeobox 1G antibody Homeobox A9 antibody Homeobox protein Hox-1.7 antibody Homeobox protein Hox-1G antibody Homeobox protein Hox-A9 antibody Homeodomain protein HOXA9 antibody Hox-1.7 antibody Hox-1.7 mouse homolog of antibody HOX1 antibody Hox1.7 antibody Hox1G antibody Hox1r5 antibody Hoxa-9 antibody Hoxa7 antibody HOXA9 antibody HOXA9/MSI2 fusion gene included antibody HOXA9/NUP98 fusion gene included antibody HXA9_HUMAN antibody MGC1934 antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P31269 |
|---|
Application
Fig1: Western blot analysis of HOXA9 on human HOXA9 recombinant protein using anti-HOXA9 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HOXA9 on HEK293 (1) and HOXA9-hIgGFc transfected HEK293 (2) cell lysate using anti-HOXA9 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining HOXA9 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with HOXA9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human HOXA9 recombinant protein, HOXA9-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
