Application
Fig1: Western blot analysis of PRDM5 on human PRDM5 recombinant protein using anti-PRDM5 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PRDM5 on HEK293 (1) and PRDM5-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM5 antibody at 1/1,000 dilution.Application
Fig3: ICC staining PRDM5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with PRDM5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PRDM5 [12C-D4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCS2 antibody PFM 2 antibody PFM2 antibody PR domain containing 5 antibody PR domain containing protein 5 antibody PR domain zinc finger protein 5 antibody PR domain-containing protein 5 antibody PRDM 5 antibody PRDM5 antibody PRDM5 protein antibody PRDM5_HUMAN antibody |
| Product description | A cDNA of PRDM5 was isolated based upon its homology to the PR domain of PRDM2. The gene encodes an open reading frame of 630 amino acids and contains a PR domain in the NH-terminal region followed by 16 zinc finger motifs. Through radiation hybrid analysis, PRDM5 was mapped to human chromosome 4q27, a region thought to contain tumor suppressor genes for ovarian, breast, lung, liver, colon, and other cancers. The gene has a CpG island promoter and is silenced in human breast, ovarian, and liver cancers. Upon infection of tumor cells, a recombinant adenovirus expressing PRDM5 causes G2/M arrest and apoptosis, suggesting that inhibition of PRDM5 may be involved in carcinogenesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | BCS2 antibody PFM 2 antibody PFM2 antibody PR domain containing 5 antibody PR domain containing protein 5 antibody PR domain zinc finger protein 5 antibody PR domain-containing protein 5 antibody PRDM 5 antibody PRDM5 antibody PRDM5 protein antibody PRDM5_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PRDM5 on human PRDM5 recombinant protein using anti-PRDM5 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PRDM5 on HEK293 (1) and PRDM5-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM5 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PRDM5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with PRDM5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PRDM5 recombinant protein, PRDM5-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
