Application
Fig1: Western blot analysis of BMP4 on human BMP4 recombinant protein using anti-BMP4 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of BMP4 on HEK293 (1) and BMP4-hIgGFc transfected HEK293 (2) cell lysate using anti-BMP4 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of BMP4 on A549 (1), HepG2 (2), and C6 (3) cell lysate using anti-BMP4 antibody at 1/1,000 dilution.Application
Fig4: ICC staining BMP4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with BMP4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | BMP4 [1C-17] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | zgc:100779 antibody BMP 2B antibody BMP 4 antibody BMP-2B antibody BMP-4 antibody BMP2B antibody BMP2B1 antibody BMP4 antibody BMP4_HUMAN antibody Bone morphogenetic protein 2B antibody Bone morphogenetic protein 4 antibody DVR4 antibody MCOPS6 antibody MGC100779 antibody OFC11 antibody zbmp-4 antibody ZYME antibody |
| Product description | Tumor growth factor, or TGFβ, is the prototypic member of a family of secreted proteins that regulate cellular proliferation and differentiation. Related proteins include the activins and the bone morphogenic proteins or BMPs. Like TGFβ, the BMPs signal through a heteromeric receptor complex (TGFβ R) composed of type I (TGFβ RI) and type II (TGFβ RII) receptors. Both the type I and the type II receptors contain an intrinsic serine/threonine kinase activity. Although signaling downstream of the TGF β R is poorly understood, several proteins have been implicated. Six TGFβ/BMP effector proteins, designated Smad1-6, may function as tumor suppressors. Smad proteins have been suggested to be transcription factors, acting similarly to the Stat family which associates directly with activated receptors and then translocates to the nucleus. Evidence supporting this assertion is drawn from the observation that Smad3 physically associates with the TGFβ R complex and that Smad1 is translocated to the nucleus 30-60 minutes after the addition of BMP-4. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:200-1:1,000 ICC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | zgc:100779 antibody BMP 2B antibody BMP 4 antibody BMP-2B antibody BMP-4 antibody BMP2B antibody BMP2B1 antibody BMP4 antibody BMP4_HUMAN antibody Bone morphogenetic protein 2B antibody Bone morphogenetic protein 4 antibody DVR4 antibody MCOPS6 antibody MGC100779 antibody OFC11 antibody zbmp-4 antibody ZYME antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | P12644 |
|---|
Application
Fig1: Western blot analysis of BMP4 on human BMP4 recombinant protein using anti-BMP4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of BMP4 on HEK293 (1) and BMP4-hIgGFc transfected HEK293 (2) cell lysate using anti-BMP4 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of BMP4 on A549 (1), HepG2 (2), and C6 (3) cell lysate using anti-BMP4 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining BMP4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with BMP4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human BMP4 recombinant protein, BMP4-hIgGFc transfected HEK293 cell lysate, A549, HepG2, C6, Hela. |
|---|---|
| Application Notes | WB:1:200-1:1,000 ICC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
