Application
Fig1: Western blot analysis of SIRT3 on human SIRT3 recombinant protein using anti-SIRT3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of SIRT3 on HEK293 (1) and SIRT3-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of SIRT3 on PANC-1 cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.Application
Fig4: Flow cytometric analysis of Hela cells with SIRT3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | SIRT3 [2A-1B] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hSIRT 3 antibody hSIRT3 antibody Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody NAD dependent deacetylase sirtuin 3 mitochondrial antibody NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody Regulatory protein SIR2 homolog 3 antibody Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody Sir 2 like 3 antibody SIR 2 like protein 3 antibody SIR 3 antibody SIR2 L3 antibody Sir2 like 3 antibody SIR2 like protein 3 antibody SIR2-like protein 3 antibody SIR2L3 antibody SIR3_HUMAN antibody SIRT 3 antibody SIRT3 antibody Sirtuin 3 antibody Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody Sirtuin type 3 antibody Sirtuin3 antibody |
| Product description | The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | hSIRT 3 antibody
hSIRT3 antibody
Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
NAD dependent deacetylase sirtuin 3 mitochondrial antibody
NAD-dependent protein deacetylase sirtuin-3 mitochondrial antibody Regulatory protein SIR2 homolog 3 antibody Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody Sir 2 like 3 antibody SIR 2 like protein 3 antibody SIR 3 antibody SIR2 L3 antibody Sir2 like 3 antibody SIR2 like protein 3 antibody SIR2-like protein 3 antibody SIR2L3 antibody SIR3_HUMAN antibody SIRT 3 antibody SIRT3 antibody Sirtuin 3 antibody Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody Sirtuin type 3 antibody Sirtuin3 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Mitochondrion matrix. |
| SwissProt ID | Q9NTG7 |
|---|
Application
Fig1: Western blot analysis of SIRT3 on human SIRT3 recombinant protein using anti-SIRT3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SIRT3 on HEK293 (1) and SIRT3-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of SIRT3 on PANC-1 cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.
Application
Fig4: Flow cytometric analysis of Hela cells with SIRT3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SIRT3 recombinant protein, SIRT3-hIgGFc transfected HEK293 cell lysate, PANC-1, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
