Application
Fig1: Western blot analysis of RAD23A on human RAD23A recombinant protein using anti-RAD23A antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RAD23A on HEK293 (1) and RAD23A)-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD23A antibody at 1/1,000 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RAD23A antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD23A antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with RAD23A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RAD23A [3E-4F] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hHR 23A antibody hHR23A antibody HR23A antibody MGC111083 antibody RAD 23a antibody RAD23 homolog A (S. cerevisiae) antibody RAD23 homolog A antibody RAD23 yeast homolog A antibody RAD23A antibody RD23A_HUMAN antibody UV excision repair protein RAD23 antibody UV excision repair protein RAD23 homolog A antibody |
| Product description | Mammalian cells express two Rad23 (genome repair protein) homologs, Rad23A (also designated HR23A) and Rad23B (also designated HR23B). In typical cells, mouse Rad23B is approximately ten times more abundant than mouse Rad23A. Endogenous XPC (xeroderma pigmentosum C protein) located in wildtype mouse embryonic fibroblasts is relatively stable; its steady-state level and stability appear to be significantly reduced by a targeted interruption of the mouse Rad23B gene, but not by that of mouse Rad23A. Loss of both mouse Rad23 genes causes a strong further reduction of the XPC protein level. The RAD23 genes (RAD23A and RAD23B), which encode the human Rad23 proteins, are crucial for excision-repair of UV-damaged DNA. RAD23 genes resemble the other DNA repair genes, RAD2, RAD6, RAD7, RAD18 and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis. Rad23 is a nuclear protein containing an ubiquitin-like domain required for biological functions. The protein, which is highly conserved, is involved in nucleotide excision repair (NER) that associates with the proteasome via its N-terminus. The C-terminal ubiquitin-associated domain of Rad23 is evolutionarily conserved from yeast to humans. Rad23 may also act as a regulator for the activity of the 26S Proteasome. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | hHR 23A antibody hHR23A antibody HR23A antibody MGC111083 antibody RAD 23a antibody RAD23 homolog A (S. cerevisiae) antibody RAD23 homolog A antibody RAD23 yeast homolog A antibody RAD23A antibody RD23A_HUMAN antibody UV excision repair protein RAD23 antibody UV excision repair protein RAD23 homolog A antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P54725 |
|---|
Application
Fig1: Western blot analysis of RAD23A on human RAD23A recombinant protein using anti-RAD23A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAD23A on HEK293 (1) and RAD23A)-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD23A antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RAD23A antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD23A antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with RAD23A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RAD23A recombinant protein, RAD23A-hIgGFc transfected HEK293 cell lysate, Hela, human bladder cancer tissue, human esophageal cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
