Application
Fig1: Western blot analysis of FBXL10 on human FBXL10 recombinant protein using anti-FBXL10 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of FBXL10 on HEK293 (1) and FBXL10-hIgGFc transfected HEK293 (2) cell lysate using anti-FBXL10 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of FBXL10 on A549 cell lysate using anti-FBXL10 antibody at 1/1,000 dilution.Application
Fig4: Flow cytometric analysis of Hela cells with FBXL10 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | FBXL10 [6G-11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Histone-H3]-lysine-36 demethylase 1B antibody CXXC-type zinc finger protein 2 antibody CXXC2 antibody F box and leucine rich repeat protein 10 antibody F box protein FBL10 antibody F-box and leucine-rich repeat protein 10 antibody F-box protein FBL10 antibody F-box/LRR-repeat protein 10 antibody Fbl10 antibody FBXL10 antibody JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody JHDM1B antibody JmjC domain-containing histone demethylation protein 1B antibody Jumonji C domain containing histone demethylase 1B antibody Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody kdm2b antibody KDM2B_HUMAN antibody Lysine (K) specific demethylase 2B antibody Lysine-specific demethylase 2B antibody PCCX2 antibody Protein containing CXXC domain 2 antibody Protein JEMMA antibody Protein-containing CXXC domain 2 antibody |
| Product description | FBXL10 (F-box and leucine-rich repeat protein 10), also known as Fbl10, CXXC2 (CXXC-type zinc finger protein 2), PCCX2, KDM2B, JEMMA (jumonji domain, EMSY-interactor, methyltransferase motif protein) or JHDM1B (jumonji C domain-containing histone demethylase 1B), is a nuclear protein that contains one F-box domain, a CXXC-type zinc finger, one JMJC domain, four leucine-rich repeats and one PHD-type zinc finger. FBXL10 belongs to the Fbls class of F-box proteins that contain leucine-rich repeats in addition to their F-box motif. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. In addition to its role in substrate recognition as a component of the E3 complex, FBXL10 functions as a dimethylation-specific demethylase, binding iron as a cofactor and demethylating lysine-36 of Histone H3. This suggests that FBXL10 plays a central role in the histone code. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | [Histone-H3]-lysine-36 demethylase 1B antibody CXXC-type zinc finger protein 2 antibody CXXC2 antibody F box and leucine rich repeat protein 10 antibody F box protein FBL10 antibody F-box and leucine-rich repeat protein 10 antibody F-box protein FBL10 antibody F-box/LRR-repeat protein 10 antibody Fbl10 antibody FBXL10 antibody JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody JHDM1B antibody JmjC domain-containing histone demethylation protein 1B antibody Jumonji C domain containing histone demethylase 1B antibody Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody kdm2b antibody KDM2B_HUMAN antibody Lysine (K) specific demethylase 2B antibody Lysine-specific demethylase 2B antibody PCCX2 antibody Protein containing CXXC domain 2 antibody Protein JEMMA antibody Protein-containing CXXC domain 2 antibody |
|---|---|
| Molecular Weight(MW) | 152 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q8NHM5 |
|---|
Application
Fig1: Western blot analysis of FBXL10 on human FBXL10 recombinant protein using anti-FBXL10 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of FBXL10 on HEK293 (1) and FBXL10-hIgGFc transfected HEK293 (2) cell lysate using anti-FBXL10 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of FBXL10 on A549 cell lysate using anti-FBXL10 antibody at 1/1,000 dilution.
Application
Fig4: Flow cytometric analysis of Hela cells with FBXL10 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human FBXL10 recombinant protein, FBXL10-hIgGFc transfected HEK293 cell lysate, A549, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
