Application
Fig1: Western blot analysis of CBX2 on human CBX2 recombinant protein using anti-CBX2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CBX2 on HEK293 (1) and CBX2-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CBX2 on different cell lysate using anti-CBX2 antibody at 1/1,000 dilution. Positive control： Line1: HUVEC Line2: HEK293 Line3: Hela Line4: NIH/3T3 Line5: A431Application
Fig4: ICC staining CBX2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-CBX2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti-CBX2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CBX2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CBX2 [C11-B10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cbx 2 antibody CBX2 antibody CBX2_HUMAN antibody CDC A6 antibody CDCA6 antibody Cell division cycle associated 6 antibody Cell division cycle associated6 antibody chromobox homolog 2 (Pc class homolog, Drosophila) antibody Chromobox homolog 2 antibody Chromobox homolog2 antibody Chromobox protein homolog 2 antibody hromobox homolog 2 antibody M33 antibody M33 polycomb like antibody MOD 2 antibody MOD2 antibody Modifier 3 antibody Modifier3 antibody Pc class homolog antibody Polycomb antibody SRXY5 antibody |
| Product description | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:200-1:1,000 IHC:1:200-1:500 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Cbx 2 antibody
CBX2 antibody
CBX2_HUMAN antibody
CDC A6 antibody
CDCA6 antibody
Cell division cycle associated 6 antibody
Cell division cycle associated6 antibody
chromobox homolog 2 (Pc class homolog Drosophila) antibody Chromobox homolog 2 antibody Chromobox homolog2 antibody Chromobox protein homolog 2 antibody hromobox homolog 2 antibody M33 antibody M33 polycomb like antibody MOD 2 antibody MOD2 antibody Modifier 3 antibody Modifier3 antibody Pc class homolog antibody Polycomb antibody SRXY5 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Nucleus |
Application
Fig1: Western blot analysis of CBX2 on human CBX2 recombinant protein using anti-CBX2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CBX2 on HEK293 (1) and CBX2-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CBX2 on different cell lysate using anti-CBX2 antibody at 1/1,000 dilution. Positive control： Line1: HUVEC Line2: HEK293 Line3: Hela Line4: NIH/3T3 Line5: A431
Application
Fig4: ICC staining CBX2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-CBX2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti-CBX2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CBX2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CBX2 recombinant protein, CBX2-hIgGFc transfected HEK293 cell lysate, HUVEC, HEK293, Hela, NIH/3T3,A431, MCF-7, human cervical cancer tissues, human rectum cancer tissues. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:1,000 IHC:1:200-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
