Application
Fig1: Western blot analysis of CD6 on human CD6 recombinant protein using anti-CD6 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CD6 on HEK293 (1) and CD6-hIgGFc transfected HEK293 (2) cell lysate using anti-CD6 antibody at 1/1,000 dilution.Application
Fig3: ICC staining CD6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CD6 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD6 antibody. Counter stained with hematoxylin.| Product Name | CD6 [12-D1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD_antigen=CD6 antibody CD6 antibody CD6 antigen Tp120 antibody CD6 molecule antibody CD6_HUMAN antibody FLJ44171 antibody OX52 antibody T cell differentiation antigen CD6 antibody T-cell differentiation antigen CD6 antibody T12 antibody TP120 antibody |
| Product description | CD6 is a type I transmembrane glycoprotein that is present on mature thymocytes, peripheral T cells and a subset of B cells. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T-cell activation and the size difference between the CD6 forms is due in part to differences in phosphorylation state. CD6 protein contains a 24-amino acid signal sequence, three extracellular "scavenger receptor cysteine-rich" (SRCR) domains, a membrane-spanning domain, and a 44-amino acid cytoplasmic domain. CD6 shows significant homology to CD5. CD6, which is also found in brain and B cell chronic lymphocytic leukemias, plays an important role in interactions of thymocytes with thymic epithelial cells. CD6 molecules can physically associate with the TCR/CD3 complex. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 ICC:1:200-1:500 IHC:1:200-1:500 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | CD_antigen=CD6 antibody CD6 antibody CD6 antigen Tp120 antibody CD6 molecule antibody CD6_HUMAN antibody FLJ44171 antibody OX52 antibody T cell differentiation antigen CD6 antibody T-cell differentiation antigen CD6 antibody T12 antibody TP120 antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P30203 |
|---|
Application
Fig1: Western blot analysis of CD6 on human CD6 recombinant protein using anti-CD6 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD6 on HEK293 (1) and CD6-hIgGFc transfected HEK293 (2) cell lysate using anti-CD6 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining CD6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CD6 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD6 antibody. Counter stained with hematoxylin.| Positive Control | Human CD6 recombinant protein, CD6-hIgGFc transfected HEK293 cell lysate, human breast cancer tissue, human rectum cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:200-1:500 IHC:1:200-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
