Application
Fig1: Western blot analysis of HPRT1 on human HPRT1 recombinant protein using anti-HPRT1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HPRT1 on HEK293 (1) and HPRT1-hIgGFc transfected HEK293 (2) cell lysate using anti-HPRT1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of HPRT1 on different cell lysate using anti-HPRT1 antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HPRT1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela cells with HPRT1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | HPRT1 [A7-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HGPRT antibody HGPRTase antibody HPRT 1 antibody HPRT_HUMAN antibody HPRT1 antibody Hypoxanthine guanine phosphoribosyltransferase antibody Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody Hypoxanthine phosphoribosyltransferase 1 antibody Hypoxanthine-guanine phosphoribosyltransferase antibody |
| Product description | HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | HGPRT antibody HGPRTase antibody HPRT 1 antibody HPRT_HUMAN antibody HPRT1 antibody Hypoxanthine guanine phosphoribosyltransferase antibody Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody Hypoxanthine phosphoribosyltransferase 1 antibody Hypoxanthine-guanine phosphoribosyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P00492 |
|---|
Application
Fig1: Western blot analysis of HPRT1 on human HPRT1 recombinant protein using anti-HPRT1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HPRT1 on HEK293 (1) and HPRT1-hIgGFc transfected HEK293 (2) cell lysate using anti-HPRT1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of HPRT1 on different cell lysate using anti-HPRT1 antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HPRT1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela cells with HPRT1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human HPRT1 recombinant protein, HPRT1-hIgGFc transfected HEK293 cell lysate, Hela, A431, A549, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
