Application
Fig1: Western blot analysis of CTNNBL1 on human CTNNBL1 recombinant protein using anti-CTNNBL1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CTNNBL1 on HEK293 (1) and CTNNBL1-hIgGFc transfected HEK293 (2) cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CTNNBL1 on different cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: Jurkat Line3: HEK293 Line4: A431 Line5: HepG2 Line6: RajiApplication
Fig4: ICC staining CTNNBL1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CTNNBL1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CTNNBL1 [5E-4F] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5730471K09Rik antibody Beta catenin like 1 antibody Beta catenin like protein 1 antibody Beta-catenin-like protein 1 antibody C20orf33 antibody Cadherin associated protein antibody Catenin beta like 1 antibody Chromosome 20 open reading frame 3 antibody CTBL1_HUMAN antibody CTNN protein antibody CTNNBL 1 antibody Ctnnbl1 antibody dJ633O20.1 antibody FLJ21108 antibody NAP antibody Nuclear associated protein antibody nuclear protein NAP antibody Nuclear-associated protein antibody NYD SP19 antibody OTTMUSP00000016933 antibody P14 antibody P14L antibody PP8304 antibody RP5 1118M15.1 antibody Testis development protein NYD SP19 antibody Testis development protein NYD-SP19 antibody |
| Product description | CTNNBL1 (catenin, β like 1), also known as NAP (nuclear-associated protein) or P14L, is an evolutionarily conserved protein with structural homology to members of the armadillo family, including β-catenin. CTNNBL1 is expressed in a variety of tissues with highest expression levels found in heart, spleen, testis, placenta, thyroid and skeletal muscle. Localizing to the nucleus, CTNNBL1 contains a bipartite nuclear localization signal, an acidic domain, a leucine-isoleucine zipper, an acidic domain and phosphorylation sites. Via its C-terminus, CTNNBL1 is believed to play a role in apoptosis. In addition, multiple SNPs (single nucleotide polymorphisms) in the CTNNBL1 have been associated with fat mass and body mass index (BMI), suggesting a possible role for CTNNBL1 in the development of obesity. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 5730471K09Rik antibody Beta catenin like 1 antibody Beta catenin like protein 1 antibody Beta-catenin-like protein 1 antibody C20orf33 antibody Cadherin associated protein antibody Catenin beta like 1 antibody Chromosome 20 open reading frame 3 antibody CTBL1_HUMAN antibody CTNN protein antibody CTNNBL 1 antibody Ctnnbl1 antibody dJ633O20.1 antibody FLJ21108 antibody NAP antibody Nuclear associated protein antibody nuclear protein NAP antibody Nuclear-associated protein antibody NYD SP19 antibody OTTMUSP00000016933 antibody P14 antibody P14L antibody PP8304 antibody RP5 1118M15.1 antibody Testis development protein NYD SP19 antibody Testis development protein NYD-SP19 antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q8WYA6 |
|---|
Application
Fig1: Western blot analysis of CTNNBL1 on human CTNNBL1 recombinant protein using anti-CTNNBL1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CTNNBL1 on HEK293 (1) and CTNNBL1-hIgGFc transfected HEK293 (2) cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CTNNBL1 on different cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: Jurkat Line3: HEK293 Line4: A431 Line5: HepG2 Line6: Raji
Application
Fig4: ICC staining CTNNBL1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CTNNBL1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CTNNBL1 recombinant protein, CTNNBL1-hIgGFc transfected HEK293 cell lysate, Hela, Jurkat, HEK293, A431, HepG2, Raji, MCF-7, human colon cancer tissue, human esophageal cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
