Application
Fig1: Western blot analysis of EIF2AK2 on human EIF2AK2 recombinant protein using anti- EIF2AK2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of EIF2AK2 on HEK293 (1) and EIF2AK2-hIgGFc transfected HEK293 (2) cell lysateusing using anti-FTL antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of EIF2AK2 on cells lysates using anti- EIF2AK2 antibody at 1/1,000 dilution. Positive control： Line1: A431 Line2: THP-1 Line3: MCF-7 Line4: PC-12Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- EIF2AK2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissuesusing anti-EIF2AK2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with EIF2AK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | EIF2AK2 [3A-H10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Double stranded RNA activated protein kinase; antibody E2AK2_HUMAN antibody eIF-2A protein kinase 2 antibody EIF2AK1 antibody EIF2AK2 antibody Eukaryotic translation initiation factor 2 alpha kinase 2 antibody Eukaryotic translation initiation factor 2-alpha kinase 2 antibody HGNC:9437 antibody Interferon induced double stranded RNA activated protein kinase antibody Interferon inducible elF2 alpha kinase antibody Interferon inducible RNA dependent protein kinase antibody Interferon-induced, double-stranded RNA-activated protein kinase antibody Interferon-inducible RNA-dependent protein kinase antibody MGC126524 antibody P1/eIF-2A protein kinase antibody P1/eIF2A protein kinase antibody p68 kinase antibody PKR antibody PPP1R83 antibody PRKR antibody Protein kinase interferon inducible double stranded RNA dependent antibody Protein kinase RNA activated antibody Protein kinase RNA-activated antibody Protein phosphatase 1 regulatory subunit 83 antibody Serine/threonine protein kinase TIK antibody Tyrosine protein kinase EIF2AK2 antibody |
| Product description | Interferon-inducible RNA-dependent protein serine/threonine kinase, PKR, is variously designated in earlier literature as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68, eIF-2α protein kinase or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA-mediated autophosphorylation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:200-1 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Double stranded RNA activated protein kinase antibody E2AK2_HUMAN antibody eIF-2A protein kinase 2 antibody EIF2AK1 antibody EIF2AK2 antibody Eukaryotic translation initiation factor 2 alpha kinase 2 antibody Eukaryotic translation initiation factor 2-alpha kinase 2 antibody HGNC:9437 antibody Interferon induced double stranded RNA activated protein kinase antibody Interferon inducible elF2 alpha kinase antibody Interferon inducible RNA dependent protein kinase antibody Interferon-induced double-stranded RNA-activated protein kinase antibody Interferon-inducible RNA-dependent protein kinase antibody MGC126524 antibody P1/eIF-2A protein kinase antibody P1/eIF2A protein kinase antibody p68 kinase antibody PKR antibody PPP1R83 antibody PRKR antibody Protein kinase interferon inducible double stranded RNA dependent antibody Protein kinase RNA activated antibody Protein kinase RNA-activated antibody Protein phosphatase 1 regulatory subunit 83 antibody Serine/threonine protein kinase TIK antibody Tyrosine protein kinase EIF2AK2 antibody |
|---|---|
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm ? perinuclear region |
| SwissProt ID | 176871 |
|---|
Application
Fig1: Western blot analysis of EIF2AK2 on human EIF2AK2 recombinant protein using anti- EIF2AK2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of EIF2AK2 on HEK293 (1) and EIF2AK2-hIgGFc transfected HEK293 (2) cell lysateusing using anti-FTL antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of EIF2AK2 on cells lysates using anti- EIF2AK2 antibody at 1/1,000 dilution. Positive control： Line1: A431 Line2: THP-1 Line3: MCF-7 Line4: PC-12
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- EIF2AK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissuesusing anti-EIF2AK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with EIF2AK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Recombinant protein, EIF2AK2-hIgGFc transfected HEK293 cell.HEK293,A431,THP-1,MCF-7,PC-12.human cervical cancer tissues, human rectum cancer tissues. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:200-1 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
