Application
Fig1: Western blot analysis of CSNK2B on human CSNK2B recombinant protein using anti-CSNK2B antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of CSNK2B on HEK293 (1) and CSNK2B-hIgGFc transfected HEK293 (2) cell lysate using anti-CSNK2B antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of CSNK2B on different cell lysates using anti-CSNK2B antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: Jurkat Line3: K562 Line4: HepG2 Line5: C6 Line6: SK-N-SH Line7: NTERA-2 Line8: MCF-7 Line9: NIH/3T3Application
Fig4: ICC staining CSNK2B (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CSNK2B antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon?cancer tissue using anti-CSNK2B antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with CSNK2B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | CSNK2B [F3-F12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Casein kinase 2 beta polypeptide antibody Casein kinase II beta subunit antibody Casein kinase II subunit beta antibody CK II beta antibody CK2B antibody CK2N antibody CSK2B antibody CSK2B_HUMAN antibody CSNK 2B antibody csnk2b antibody G5A antibody MGC138222 antibody MGC138224 antibody Phosvitin antibody Protein G5a antibody |
| Product description | Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Ie, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The a catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Casein kinase 2 beta polypeptide antibody Casein kinase II beta subunit antibody Casein kinase II subunit beta antibody CK II beta antibody CK2B antibody CK2N antibody CSK2B antibody CSK2B_HUMAN antibody CSNK 2B antibody csnk2b antibody G5A antibody MGC138222 antibody MGC138224 antibody Phosvitin antibody Protein G5a antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Chromatin, cytoplasm, cytosol, extracellular exosome, nuclear matrix, nucleoplasm,nucleus |
Application
Fig1: Western blot analysis of CSNK2B on human CSNK2B recombinant protein using anti-CSNK2B antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CSNK2B on HEK293 (1) and CSNK2B-hIgGFc transfected HEK293 (2) cell lysate using anti-CSNK2B antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CSNK2B on different cell lysates using anti-CSNK2B antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: Jurkat Line3: K562 Line4: HepG2 Line5: C6 Line6: SK-N-SH Line7: NTERA-2 Line8: MCF-7 Line9: NIH/3T3
Application
Fig4: ICC staining CSNK2B (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CSNK2B antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon?cancer tissue using anti-CSNK2B antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with CSNK2B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human CSNK2B recombinant protein, CSNK2B-hIgGFc transfected HEK293 cell lysate, Hela, Jurkat, K562, HepG2, C6, SK-N-SH, NTERA-2, MCF-7, NIH/3T3. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
