Application
Fig1: Western blot analysis of G-CSF on human G-CSF recombinant protein using anti- G-CSF antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of G-CSF on HEK293 (1) and G-CSF-hIgGFc transfected HEK293 (2) cell lysate using anti-G-CSF antibody at 1/1,000Application
Fig3: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- G-CSF antibody. Counter stained with hematoxylin.Application
Fig4: ICC staining G-CSF (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: ICC staining G-CSF (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | CSF3 [7F-4E] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C17orf33 antibody Colony stimulating factor 3 (granulocyte) antibody Colony stimulating factor 3 antibody CSF 3 antibody CSF beta antibody CSF3 antibody CSF3_HUMAN antibody CSF3OS antibody Csfg antibody Filgrastim antibody G-CSF antibody GCSA antibody GCSF antibody Granulocyte colony stimulating factor antibody Granulocyte colony-stimulating factor antibody Lenograstim antibody Macrophage granulocyte inducer 2 antibody MGC45931 antibody MGI 2 antibody Pluripoietin antibody |
| Product description | Granulocyte-colony stimulating factor, G-CSF, is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The murine G-CSF cDNA encodes a 208 amino acid precursor containing a 30 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF cDNAs, which are identical except for a three amino acid deletion in the N-terminus of one form of the protein, have been isolated from human cells. Murine and human G-CSF share 73% sequence identity at the amino acid level. G-CSF signals through the G-CSF receptor, G-CSFR, a heavily glycosylated 812 amino acid polypeptide with a single transmembrane domain. Stimulation of the G-CSFR results in the activation of the Ras/MAPK pathway and phosphorylation of the adaptor protein Shc. Studies indicate that the kinases Lyn and Syk and the transcription factor Stat3 are activated in response to G-CSF stimulation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | C17orf33 antibody Colony stimulating factor 3 (granulocyte) antibody Colony stimulating factor 3 antibody CSF 3 antibody CSF beta antibody CSF3 antibody CSF3_HUMAN antibody CSF3OS antibody Csfg antibody Filgrastim antibody G-CSF antibody GCSA antibody GCSF antibody Granulocyte colony stimulating factor antibody Granulocyte colony-stimulating factor antibody Lenograstim antibody Macrophage granulocyte inducer 2 antibody MGC45931 antibody MGI 2 antibody Pluripoietin antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P09919 |
|---|
Application
Fig1: Western blot analysis of G-CSF on human G-CSF recombinant protein using anti- G-CSF antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of G-CSF on HEK293 (1) and G-CSF-hIgGFc transfected HEK293 (2) cell lysate using anti-G-CSF antibody at 1/1,000
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- G-CSF antibody. Counter stained with hematoxylin.
Application
Fig4: ICC staining G-CSF (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining G-CSF (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | Human CSF3 recombinant protein, CSF3-hIgGFc transfected HEK293 cell lysate, human esophageal cancer tissue, A549, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
