Application
Fig1: Western blot analysis of Rab3a on human Rab3a recombinant protein using anti-Rab3a antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Rab3a on HEK293 (1) and Rab3a-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with Rab3a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | Rab3a [D1-12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rab 3A antibody RAB 3A member RAS oncogene family antibody Rab3a antibody RAB3A member RAS oncogene family antibody RAB3A_HUMAN antibody RAS associated protein RAB 3A antibody RAS associated protein RAB3A antibody Ras related protein Rab 3A antibody Ras related protein Rab3A antibody Ras-related protein Rab-3A antibody |
| Product description | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Rab 3A antibody RAB 3A member RAS oncogene family antibody Rab3a antibody RAB3A member RAS oncogene family antibody RAB3A_HUMAN antibody RAS associated protein RAB 3A antibody RAS associated protein RAB3A antibody Ras related protein Rab 3A antibody Ras related protein Rab3A antibody Ras-related protein Rab-3A antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P20336 |
|---|
Application
Fig1: Western blot analysis of Rab3a on human Rab3a recombinant protein using anti-Rab3a antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Rab3a on HEK293 (1) and Rab3a-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with Rab3a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Rab3a recombinant protein, Rab3a-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
