Application
Fig1: Western blot analysis of AIM2 on human AIM2 recombinant protein using anti-AIM2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of AIM2 on HEK293 (1) and AIM2-hIgGFc transfected HEK293 (2) cell lysate using anti-AIM2 antibody at 1/1,000 dilution.Application
Fig3: ICC staining AIM2 (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining AIM2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with AIM2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | AIM2 [4G-11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Absent in melanoma 2 antibody AIM 2 antibody Aim2 antibody AIM2_HUMAN antibody Interferon-inducible protein AIM2 antibody OTTHUMP00000035296 antibody PYHIN4 antibody |
| Product description | Interferon-inducible protein AIM2 (Absent in melanoma 2) is a 343 amino acid protein belonging to the HIN-200 family. Induced by IFN-γ, AIM2 is thought to act as a tumor suppressor by repressing NFκB transcriptional activity. Localized to the nucleus, AIM2 contains one DAPIN domain and one HIN-200 domain. The DAPIN domain is composed mostly of α-helixes and is a protein-protein interaction domain capable of binding other DAPIN domains. The HIN-200 domain has been shown to bind directly to DNA, which, along with the binding of another protein ASC, results in the activation of Caspase-1. AIM2 is present as a homodimer and is expressed highly in small intestine, testis, peripheral blood leukocytes and spleen. Defects in AIM2 are believed to be a cause of microsatellite unstable colon cancers. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Absent in melanoma 2 antibody AIM 2 antibody Aim2 antibody AIM2_HUMAN antibody Interferon-inducible protein AIM2 antibody OTTHUMP00000035296 antibody PYHIN4 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Nucleus,cytoplasm. |
| SwissProt ID | O14862 |
|---|
Application
Fig1: Western blot analysis of AIM2 on human AIM2 recombinant protein using anti-AIM2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of AIM2 on HEK293 (1) and AIM2-hIgGFc transfected HEK293 (2) cell lysate using anti-AIM2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining AIM2 (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining AIM2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with AIM2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human AIM2 recombinant protein, AIM2-hIgGFc transfected HEK293 cell lysate, A549, MCF-7, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
