Application
Fig1: Western blot analysis of RPS6KA2 on human RPS6KA2 recombinant protein using anti-RPS6KA2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RPS6KA2 on HEK293 (1) and RPS6KA2-hIgGFc transfected HEK293 (2) cell lysate using anti-RPS6KA2 antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of RPS6KA2 on different cell lysate using anti-RPS6KA2 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: A431 Line3: HEK293 Line4: Jurkat Line5: HepG2 Line6: MCF-7 Line7: NIH/3T3Application
Fig4: ICC staining RPS6KA2 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RPS6KA2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RPS6KA2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of Hela cells with RPS6KA2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RPS6KA2 [8C-4C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 90 kDa ribosomal protein S6 kinase 2 antibody HU 2 antibody KS6A2_HUMAN antibody MAP kinase activated protein kinase 1c antibody MAP kinase-activated protein kinase 1c antibody MAPK-activated protein kinase 1c antibody MAPKAP kinase 1c antibody MAPKAPK-1c antibody MAPKAPK1C antibody Mitogen.activated protein kinase-activated protein kinase 1C antibody p90 RSK3 antibody p90-RSK 2 antibody p90RSK2 antibody pp90RSK3 antibody Ribosomal protein S6 kinase alpha-2 antibody ribosomal protein S6 kinase, 90kDa, polypeptide 2 antibody Ribosomal S6 kinase 3 antibody RPS6KA2 antibody RSK 3 antibody RSK antibody RSK-3 antibody S6K alpha antibody S6K alpha 2 antibody S6K-alpha-2 antibody |
| Product description | The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase β, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 90 kDa ribosomal protein S6 kinase 2 antibody
HU 2 antibody
KS6A2_HUMAN antibody
MAP kinase activated protein kinase 1c antibody
MAP kinase-activated protein kinase 1c antibody
MAPK-activated protein kinase 1c antibody
MAPKAP kinase 1c antibody
MAPKAPK-1c antibody
MAPKAPK1C antibody
Mitogen.activated protein kinase-activated protein kinase 1C antibody
p90 RSK3 antibody
p90-RSK 2 antibody
p90RSK2 antibody
pp90RSK3 antibody
Ribosomal protein S6 kinase alpha-2 antibody
ribosomal protein S6 kinase 90kDa polypeptide 2 antibody Ribosomal S6 kinase 3 antibody RPS6KA2 antibody RSK 3 antibody RSK antibody RSK-3 antibody S6K alpha antibody S6K alpha 2 antibody S6K-alpha-2 antibody |
|---|---|
| Molecular Weight(MW) | 83 kDa |
| Cellular Localization | Nucleus, cytoplasm. |
Application
Fig1: Western blot analysis of RPS6KA2 on human RPS6KA2 recombinant protein using anti-RPS6KA2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RPS6KA2 on HEK293 (1) and RPS6KA2-hIgGFc transfected HEK293 (2) cell lysate using anti-RPS6KA2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of RPS6KA2 on different cell lysate using anti-RPS6KA2 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: A431 Line3: HEK293 Line4: Jurkat Line5: HepG2 Line6: MCF-7 Line7: NIH/3T3
Application
Fig4: ICC staining RPS6KA2 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RPS6KA2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RPS6KA2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with RPS6KA2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RPS6KA2 recombinant protein, RPS6KA2-hIgGFc transfected HEK293 cell lysate, Hela, A431, HEK293, Jurkat, HepG2, MCF-7, NIH-3T3 cell lysate, human cervical cancer tissue, human lung cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
