Application
Fig1: Western blot analysis of RBL2 on human RBL2 recombinant protein using anti-RBL2 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of RBL2 on HEK293 (1) and RBL2-hIgGFc transfected HEK293 (2) cell lysate using anti-RBL2 antibody at 1/1,000 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with RBL2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | RBL2 [8A-8C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 130 kDa retinoblastoma associated protein antibody FLJ26459 antibody p130 antibody PRB 2 antibody PRB2 antibody Rb 2 antibody Rb2 antibody RBL 2 antibody RBL2 antibody RBR 2 antibody RBR2 antibody Retinoblastoma like 2 antibody Retinoblastoma like protein 2 antibody Retinoblastoma related gene antibody |
| Product description | The human retinoblastoma gene product Rb plays an important role in the negative regulation of cell proliferation. The Rb family includes p107 and p130, which form complexes with E2F proteins, and share a high degree of structural homology in the adenovirus E1A binding domain (i.e., "pocket region"), which plays a primary role in the function of these proteins. The Rb family members undergo cell cycle dependent phosphorylation during mid-G1 to S phase transition, which is dependent upon the activity of cyclin D/cdk4. In contrast to pRb and p107, p130 is also phosphorylated during G0 and the early G1 phase of the cell cycle. p130 is specifically phosphorylated on serine and threonine residues in cells arrested in G0 by serum deprivation or density arrest, and these residues are clustered within a short co-linear region unique to p130 defined as the Loop. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 130 kDa retinoblastoma associated protein antibody FLJ26459 antibody p130 antibody PRB 2 antibody PRB2 antibody Rb 2 antibody Rb2 antibody RBL 2 antibody RBL2 antibody RBR 2 antibody RBR2 antibody Retinoblastoma like 2 antibody Retinoblastoma like protein 2 antibody Retinoblastoma related gene antibody |
|---|---|
| Molecular Weight(MW) | 128 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q08999 |
|---|
Application
Fig1: Western blot analysis of RBL2 on human RBL2 recombinant protein using anti-RBL2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RBL2 on HEK293 (1) and RBL2-hIgGFc transfected HEK293 (2) cell lysate using anti-RBL2 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with RBL2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human RBL2 recombinant protein, RBL2-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
