Application
Fig1: Western blot analysis of HSF1 on human HSF1 recombinant protein using anti-HSF1 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of HSF1 on HEK293 (1) and HSF1-hIgGFc transfected HEK293 (2) cell lysate using anti-HSF1 antibody at 1/1,000 dilution.Application
Fig3: ICC staining HSF1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-HSF1 antibody. Counter stained with hematoxylin.| Product Name | HSF1 [4D-5F] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICCIHCWB |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Heat shock factor 1 antibody Heat shock factor protein 1 antibody Heat shock transcription factor 1 antibody HSF 1 antibody hsf1 antibody HSF1_HUMAN antibody HSTF 1 antibody HSTF1 antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Nucleus, cytoplasm. |
| SwissProt ID | Q00613 |
|---|
Application
Fig1: Western blot analysis of HSF1 on human HSF1 recombinant protein using anti-HSF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of HSF1 on HEK293 (1) and HSF1-hIgGFc transfected HEK293 (2) cell lysate using anti-HSF1 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining HSF1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-HSF1 antibody. Counter stained with hematoxylin.| Positive Control | Human HSF1 recombinant protein, HSF1-hIgGFc transfected HEK293 cell lysate, A431, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
