Application
Fig1: Western blot analysis of Villin on human Villin recombinant protein using anti- Villin antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of Villin on HEK293 (1) and Villin -hIgGFc transfected HEK293 (2) cell lysate using anti- Villin antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of Villin on SW620 cell lysate using anti- Villin antibody at 1/1,000 dilution.Application
Fig4: ICC staining Villin (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Villin antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of SW620 cells with Villin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | VIL1 [G11-5E] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D2S1471 antibody OTTHUMP00000164145 antibody VIL antibody VIL1 antibody VILI_HUMAN antibody Villin 1 antibody Villin-1 antibody Villin1 antibody |
| Product description | Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | D2S1471 antibody OTTHUMP00000164145 antibody VIL antibody VIL1 antibody VILI_HUMAN antibody Villin 1 antibody Villin-1 antibody Villin1 antibody |
|---|---|
| Molecular Weight(MW) | 93 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| SwissProt ID | P09327 |
|---|
Application
Fig1: Western blot analysis of Villin on human Villin recombinant protein using anti- Villin antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Villin on HEK293 (1) and Villin -hIgGFc transfected HEK293 (2) cell lysate using anti- Villin antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Villin on SW620 cell lysate using anti- Villin antibody at 1/1,000 dilution.
Application
Fig4: ICC staining Villin (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Villin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of SW620 cells with Villin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human VIL1 recombinant protein, VIL1-hIgGFc transfected HEK293 cell lysate, SW620, Hela, human cervical cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
