Application
Fig1: Western blot analysis of UBE2C on human UBE2C recombinant protein using anti-UBE2C antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of UBE2C on HEK293 (1) and UBE2C-hIgGFc transfected HEK293 (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.Application
Fig3: Western blot analysis of UBE2C on Hela (1) and Raji (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human?liver cancer tissue using anti-UBE2C antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-UBE2C antibody. Counter stained with hematoxylin.| Product Name | UBE2C [5D-3F] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyclin selective ubiquitin carrier protein antibody dJ447F3.2 antibody Mitotic specific ubiquitin conjugating enzyme antibody UB E2C antibody UBCH 10 antibody UbcH10 antibody UBE 2C antibody Ube2c antibody UBE2C_HUMAN antibody Ubiquitin carrier protein C antibody Ubiquitin carrier protein E2 C antibody Ubiquitin carrier protein E2C antibody Ubiquitin conjugating enzyme E2 C antibody Ubiquitin conjugating enzyme E2C antibody Ubiquitin protein ligase C antibody Ubiquitin-conjugating enzyme E2 C antibody Ubiquitin-protein ligase C antibody |
| Product description | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Cyclin selective ubiquitin carrier protein antibody dJ447F3.2 antibody Mitotic specific ubiquitin conjugating enzyme antibody UB E2C antibody UBCH 10 antibody UbcH10 antibody UBE 2C antibody Ube2c antibody UBE2C_HUMAN antibody Ubiquitin carrier protein C antibody Ubiquitin carrier protein E2 C antibody Ubiquitin carrier protein E2C antibody Ubiquitin conjugating enzyme E2 C antibody Ubiquitin conjugating enzyme E2C antibody Ubiquitin protein ligase C antibody Ubiquitin-conjugating enzyme E2 C antibody Ubiquitin-protein ligase C antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Cytoplasm, cytosol, nucleoplasm , plasma membrane. |
| SwissProt ID | O00762 |
|---|
Application
Fig1: Western blot analysis of UBE2C on human UBE2C recombinant protein using anti-UBE2C antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of UBE2C on HEK293 (1) and UBE2C-hIgGFc transfected HEK293 (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of UBE2C on Hela (1) and Raji (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human?liver cancer tissue using anti-UBE2C antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-UBE2C antibody. Counter stained with hematoxylin.| Positive Control | Human UBE2C recombinant protein, UBE2C-hIgGFc transfected HEK293 cell lysate, Hela and Raji cell lysate, human?liver cancer tissue, human esophageal cancer tissue |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
