Application
Fig1: Western blot analysis of AP2 alpha on human AP2 alpha recombinant protein using anti- AP2 alpha antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of AP2 alpha on HEK293 (1) and AP2 alpha -hIgGFc transfected HEK293 (2) cell lysate using anti- AP2 alpha antibody at 1/1,000 dilution.Application
Fig3: ICC staining AP2 alpha (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of Hela cells with AP2 alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | TFAP2A [10-C5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating enhancer binding protein 2 alpha antibody Activating enhancer-binding protein 2-alpha antibody Activator protein 2 antibody AP 2 transcription factor antibody AP 2alpha antibody AP-2 antibody AP-2 transcription factor antibody AP2 antibody AP2 Transcription Factor antibody AP2-alpha antibody AP2A_HUMAN antibody AP2TF antibody BOFS antibody FLJ51761 antibody TFAP 2 antibody TFAP 2A antibody TFAP2 antibody TFAP2A antibody Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody Transcription factor AP-2-alpha antibody Transcription factor AP2 alpha antibody |
| Product description | AP-2 transcription factor family members include AP-2α, AP-2β and AP-2γ, which specifically bind to the DNA consensus sequence CCCCAGGC and initiate transcription of selected genes. AP-2, also known as ERF-1, plays a role in regulating estrogen receptor expression. AP-2β, a splice variant of AP-2α, inhibits AP-2 activity. Besides subscribing to the AP-2 complex, AP-2α, AP-2β and AP-2γ proteins compose the OB2-1 transcription factor complex. OB2-1 specifically upregulates expression of the proto-oncogene c-ErbB-2, which is overexpressed in 25-30% of breast cancers. AP-2α may play an important role in the development of ectodermal-derived tissues. Deleterious mutations involving the AP-2α gene are linked to microphthalmia, corneal clouding and other anterior eye chamber defects. The ubiquitously expressed AP-4 transcription factor specifically binds to the DNA consensus sequence 5'-CAGCTG-3'. AP-4 interacts with promoters for immunoglobulin-κ gene families and simian virus 40. AP-4 may enhance the transcription of the human Huntington's disease gene. AP-4 is a helix-loop-helix protein that contains two distinctive leucine repeat elements. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Activating enhancer binding protein 2 alpha antibody Activating enhancer-binding protein 2-alpha antibody Activator protein 2 antibody AP 2 transcription factor antibody AP 2alpha antibody AP-2 antibody AP-2 transcription factor antibody AP2 antibody AP2 Transcription Factor antibody AP2-alpha antibody AP2A_HUMAN antibody AP2TF antibody BOFS antibody FLJ51761 antibody TFAP 2 antibody TFAP 2A antibody TFAP2 antibody TFAP2A antibody Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody Transcription factor AP-2-alpha antibody Transcription factor AP2 alpha antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P05549 |
|---|
Application
Fig1: Western blot analysis of AP2 alpha on human AP2 alpha recombinant protein using anti- AP2 alpha antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of AP2 alpha on HEK293 (1) and AP2 alpha -hIgGFc transfected HEK293 (2) cell lysate using anti- AP2 alpha antibody at 1/1,000 dilution.
Application
Fig3: ICC staining AP2 alpha (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of Hela cells with AP2 alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human AP2 alpha recombinant protein, AP2 alpha -hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
