Application
Fig1: Western blot analysis of PER3 on human PER3 recombinant protein using anti-PER3 antibody at 1/1,000 dilution.Application
Fig2: Western blot analysis of PER3 on HEK293 (1) and PER3-hIgGFc transfected HEK293 (2) cell lysate using anti-PER3 antibody at 1/1,000 dilution.Application
Fig3: ICC staining PER3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PER3 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PER3 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with PER3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Product Name | PER3 [9B-7D] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2810049O06Rik antibody Cell growth inhibiting gene 13 protein antibody Cell growth-inhibiting gene 13 protein antibody Circadian clock protein PERIOD 3 antibody GIG13 antibody gPER3 antibody Growth inhibiting protein 13 antibody HGNC:8847 antibody hPER3 antibody mPer3 antibody Per3 antibody PER3_HUMAN antibody Period 3 antibody Period circadian clock 3 antibody Period circadian protein 3 antibody Period circadian protein homolog 3 antibody Period homolog 3 (Drosophila) antibody Period homolog 3 antibody Period homolog 3, Drosophila antibody Period3 antibody |
| Product description | Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. A number of transcription factors appearing to be molecular components of the SCN clock have been identified. Mutations within the Clock gene increase the length of the endogenous period and cause a loss of rhythmicity of circadian oscillations. Three mammalian period proteins, designated Per1, Per2 and Per3, exhibit circadian rhythyms in the SCN. During subjective night, Per1 and Per2 RNA levels increase in response to light pulses while Per3 RNA levels show no change in response to light pulses. Tim, for timeless, interacts with Per1 as well as Per2; and Tim and Per1 negatively regulate Clock-BMAL1-induced transcription. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 2810049O06Rik antibody
Cell growth inhibiting gene 13 protein antibody
Cell growth-inhibiting gene 13 protein antibody
Circadian clock protein PERIOD 3 antibody
GIG13 antibody
gPER3 antibody
Growth inhibiting protein 13 antibody
HGNC:8847 antibody
hPER3 antibody
mPer3 antibody
Per3 antibody
PER3_HUMAN antibody
Period 3 antibody
Period circadian clock 3 antibody
Period circadian protein 3 antibody
Period circadian protein homolog 3 antibody
Period homolog 3 (Drosophila) antibody
Period homolog 3 antibody
Period homolog 3 Drosophila antibody Period3 antibody |
|---|---|
| Molecular Weight(MW) | 132 kDa |
| Cellular Localization | Cytoplasm, nucleus |
| SwissProt ID | P56645 |
|---|
Application
Fig1: Western blot analysis of PER3 on human PER3 recombinant protein using anti-PER3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PER3 on HEK293 (1) and PER3-hIgGFc transfected HEK293 (2) cell lysate using anti-PER3 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining PER3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PER3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PER3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with PER3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PER3 recombinant protein, PER3-hIgGFc transfected HEK293 cell lysate, Hela, human ovarian cancer tissue, human rectum cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
